【摘要】 选用60只雄性断奶仔兔随机分为4组,对照组饲喂基础日粮,PAS38组在基础日粮中添加0.1%腊样芽孢杆菌PAS38制剂,甘露聚糖组在基础日粮中添加0.2%β-甘露聚糖制剂,菌糖混合组在基础日粮中分别添加0.1%PAS38制剂和0.2%β-甘露聚糖制剂.家兔饲喂至第15、30、45天时每组宰杀5只,采用免疫组织化学SABC法检测家兔小肠中生长抑素(SS)、5-羟色胺(5-HT)阳性细胞数量和表达.结果表明:PAS38制剂和甘露聚糖制剂均减少家兔小肠中SS阳性细胞的数量和降低SS细胞的表达强度,增加小肠中5-HT阳性细胞数量和提高5-HT细胞的表达强度;两者联合应用效果更加明显,与对照组相比具有显著或极显著差异(P<0.05或P<0.01),表现出一定的协同效应.更多还原

【Abstract】 Sixty male weaned rabbits were randomly divided into four groups: control group was fed the basic diet;PAS38 group was fed diet supplemented with 0.1% B.cereus PAS38;mannosan group was fed diet supplemented with 0.2% mannosan;and mix group was fed diet supplemented with 0.1% B.cereus PAS38 and 0.2% mannosan.Immunohistochemistry SABC was used to measure 5-HT and somatostatin(SS) of immunoreactive cells and the expression in small intestine of rabbit on the 15,30 and 45th day.The results show that the additive of PAS38 or β-mannan could decrease the number of immunoreactive cells of SS and the expression of SS,and increase the number of immunoreactive cells of 5-HT and the expression of 5-HT in small intestine of rabbit.United application of two kinds of additives showed obviously efficacious(P<0.05 or P<0.01),compared with control group.The results above indicated that combined application of B.cereus PAS38 and β-mannan preparation could achieve good synergistic effect on modulating intestinal endocrine-immunity of rabbit.更多还原