【摘要】 研究哈茨木霉TS1与多菌灵复合使用对水稻幼苗的诱导抗病性及对立枯病的防治效果.水稻种子经哈茨木霉TS1与多菌灵复合处理后,水稻幼苗根部的过氧化物酶(peroxidase)、苯丙氨酸解氨酶(phenylalanine ammonialyase)、多酚氧化酶(polyphenol oxidase)及超氧化物歧化酶(superoxide dismutase)活性均高于对照;复合处理对立枯病的室内防效达82.25%,比哈茨木霉TS1和多菌灵单独处理分别提高26.87%和9.90%.田间实验表明,菌药3种配比处理对立枯病的防效均大于单独处理,其中以6∶4配比的防效最高,较单独处理分别提高44.46%和11.66%,差异显著;经复合处理的水稻秧苗在株高、根长、百株鲜重和百株干重上均显著高于对照;哈茨木霉TS1可以在水稻幼苗根部定殖,每克根的根际和根表定殖量分别为104和103个以上,不同菌药配比对根际和根表定殖量均无显著影响.更多还原

【Abstract】 Control efficacies of Trichoderma harzianum TS1 in combination with carbendazim against rice seeding blight pathogens were investigated,and induced resistance of rice to the disease was conducted. Activities of POD,PAL,PPO and SOD in root extracts from T. harzianum TS1 plus carbendazim treated seed plots were higher than those from untreated control. Simultaneously,seed treated with this combination counted to 82.25% disease control in growth chamber,which was 26.87% and 9.90% higher than that resulted by treatment with T. harzianum TS1 and carbendazim alone. Field efficacies of combinations at three ratios exceeded the efficacies of the two agents used separately,indicating possible synergistic effects. Significant synergistic effect was observed in combination at ratio of 6 to 4,manifested by 44.46% and 11.66% higher efficacy than each agent. Seed treated with each combination resulted in a significant increase in shoot height,root length,total wet and dry weight compared with untreated control. T. harzianum TS1 had the ability to colonize the rhizosphere and rhizoplane of rice plants with mean population sizes above 104 and 103 cfu·g-1 fresh root,no significant treatment effects on colonization rate were found.更多还原