【摘要】 将源于枯草芽孢杆菌168的木糖启动子xylR和来源于载体pGFPuv的gfp基因连接,插入枯草芽孢杆菌—大肠杆菌穿梭载体pIM,成功构建了以绿色荧光蛋白(GFP)为报告基因的重组载体pIM-GFP。转化枯草芽孢杆菌菌株BS523,突变株在荧光显微镜及紫外灯下均观察到较强的绿色荧光,同时PCR鉴定结果正确。表明重组载体pIM-GFP成功转入菌株BS523,并且gfp基因成功表达。更多还原

【Abstract】 The xylR promoter from B.subtilis 168 chromosomal DNA was ligated with GFP gene from plasmid GFPuv.The expression fragment xylR-GFP was inserted into the shuttle-vector pIM.The recombinant vector pIM-GFP was successfully obtained.The pIM-GFP was transformed into BS523 strain.Transformants were identified by green fluorescence observation with microscope.The results showed that pIM-GFP was successfully integrated into the strain BS523.更多还原