【摘要】 以茎段为外植体,建立了报春石斛Dendrobium primulinum的离体培养再生体系。结果表明:愈合组织诱导以培养基MS(Murashige and Skoog)+5.0mg·L-16-苄氨基嘌呤(6-BA)+1.5mg·L-12,4-D为最优,诱导率达33.33%;分化和增殖培养基均以MS+1.0mg·L-16-BA+0.1mg·L-1萘乙酸(NAA)+体积分数10%椰子水为佳,分化率最高可达83.20%,增殖倍数达3.82,外源物质椰子水可促进不定芽的形成及增殖。形成的无根苗转移到1/2MS+0.5mg·L-16-BA+1.0mg·L-1NAA+0.5g·L-1活性炭培养基上诱导生根发育形成完整植株。图1表4参12更多还原

【Abstract】 An in vitro plant regeneration system of Dendrobium primulinum was established using stem sections as explants. Results showed that the optimum callus inducing medium was Murashige and Skoog (MS) + 5.0 mg·L-1 6-BA + 1.5 mg·L-1 2,4-D with an induction rate of 33.3%. Adventitious buds were effectively generated and proliferated on MS + 1.0 mg·L-16-BA + 0.1 mg·L-1 NAA + coconut water 10% with a differentiation rate of 83.2% and proliferation of 3.82 times. Exogenous coconut water promoted adventitious bud generation and proliferation. The best rooting medium was 1/2MS + 0.5 mg·L-1 6-BA +1.0 mg·L-1NAA + 0.5 g·L-1 activated carbon. [Ch,1 fig. 4 tab. 12 ref.]更多还原