【摘要】 采用ISSR(inter simple sequence repeats)分子标记技术研究红晶李Prunus salicina‘Hongjing’与其他19个李主栽品种的亲缘关系,揭示红晶李的遗传基础。从120个ISSR引物中筛选出24个具有多态性的引物,共检测到93条清晰的条带,其中84条具有多态性,占90.3%。通过POPGENE 32软件分析,红晶李与其他19个李品种间的遗传距离为0.215 5～0.734 8,其中与大石早生P.salicina‘Oishi Wase’间的遗传距离最近,遗传距离仅为0.215 5,而与槜李P.salicina‘Zuili’间的遗传距离最大,遗传距离为0.734 8。在本实验条件下,引物U811在1 000 bp左右有一条红晶李的特有条带,可作为它的特征带,表明红晶李具有其特有的遗传基础。更多还原

【Abstract】 Prunus salicina ‘Hongjing’ is a new cultivar selected by us,which early maturing,scarlet,and having good taste.The relationships between P.salicina ‘Hongjing’ and other main plum cultivars were studied using the ISSR(inter simple sequence repeats) technique.Altogether,120 primers were screened,from which 24 polymorphism primers were selected.Using these primers 98 bands were harvested,of which 84 polymorphism loci were identified.POPGENE 32 was used to calculate the genetic distance between cultivars.Results showed that the polymorphism ratio was 90.3%,and the genetic distance between P.salicina ‘ Hongjing’ and 19 other cultivars ranged from 0.215 5(for ‘ Oishi Wase’) to 0.734 8(for ‘Zuili’).Thus,one 1 000 bp band,amplified with primer U811,could serve as the characteristic band of P.salicina ‘Hongjing’.This study showed that P.salicina ‘Hongjing’ had its own genetic basis.更多还原