【摘要】 目的分析小鼠脾细胞中CD4+CD25+Foxp3+T细胞在日本血吸虫感染后的动态变化,探讨CD4+CD25+Foxp3+T细胞与血吸虫感染的关系。方法常规逸出尾蚴,感染小鼠24只,随机分为4组,同时设立阴性对照。感染后第3、6、9、12周分批处死小鼠,取脾脏制备单细胞悬液,用FITC-CD4和PE-CD25单抗进行细胞表面染色,洗涤后,经打孔液和固定液处理,再用PE-cy5-Foxp3抗体进行核内染色,流式细胞仪检测CD4+CD25+Foxp3+调节性T细胞的变化。结果小鼠感染血吸虫后第3、6、9、12周脾细胞中CD4+CD25+Foxp3+T细胞百分率分别为(2.57±0.13)%、(1.77±0.21)%、(1.10±0.05)%和(0.83±0.04)%,呈逐步下降趋势(P<0.05);CD4+CD25-Foxp3+T细胞百分率分别为(0.25±0.007)%、(0.27±0.13)%、(0.38±0.15)%和(0.35±0.07)%,差异无统计学意义(P>0.05)。未感染小鼠脾细胞中CD4+CD25+Foxp3+T细胞百分率分别为(2.63±0.09)%、(3.13±0.47)%、(2.78±0.19)%和(2.82±0.12)%,差异无统计学意义(P>0.05);CD4+CD25-Foxp3+T细胞百分率分别为(0.35±0.10)%、(0.43±0.13)%、(0.30±0.08)%和(0.31±0.09)%,差异无统计学意义(P>0.05)。感染组与未感染组相比从第6周开始脾细胞中CD4+CD25+Foxp3+T细胞百分率显著下降(P<0.05),CD4+CD25-Foxp3+T细胞百分率差异无统计学意义(P>0.05)。结论CD4+CD25+Foxp3+T细胞在日本血吸虫感染中具有免疫抑制作用,与血吸虫虫卵肉芽肿慢性病变具有相关性。更多还原

【Abstract】 Objective To analyze the dynamics of CD4+ CD25+ Foxp3+ T cells in spleen cells of mice infected with cercariae of Schistosoma japonicum and investigate the relationship between CD4+ CD25+ Foxp3+ T cells and the infection of S.japonicum Methods Twenty-four mice were infected by cercariae and divided into 4 groups,and 24 mice were uninfected.At 3,6,9,and 12 weeks after infection,the mice were sacrificed.A spleen single cell suspension was prepared and flow cytometry analysis was performed on the CD4+ CD25+ Foxp3+ T cells of spleen cells stained with anti-FITC-CD4,PE-CD25,or PE-CY5-FOXP3 monoclonal antibody.Results At 3,6,9,and 12 weeks,the percentage of CD4+ CD25+ Foxp3+ T cells in infected mice was(2.57±0.13)%,(1.77±0.21)%,(1.10±0.05)%,and(0.83±0.04)%,indicating a gradual decline(P<0.05).The percentage of CD4+ CD25-Foxp3+ T cells were(0.25±0.007)%,(0.27±0.13)%,(0.38±0.15)%,and(0.35±0.07)%,indicating that the difference was not statistically significant(P>0.05).For uninfected mice,the percentage of CD4+ CD25+ Foxp3+ T cells was(2.63±0.09)%,(3.13±0.47)%,(2.78±0.19)%,and(2.82±0.12)%(P>0.05),and the percentage of CD4+ CD25-Foxp3+ T cells was(0.35±0.10)%,(0.43±0.13)%,(0.30±0.08)%,and(0.31±0.09)%(P>0.05);differences in both were not statistically significant.Compared to uninfected mice,the CD4+ CD25+ Foxp3+ T cells in infected mice declined markedly 6 weeks after infection(P<0.05),but the difference in CD4+ CD25-Foxp3+ T cells was not statistically significant(P>0.05).Conclusion This study suggested that CD4+ CD25+ Foxp3+ T cells act to suppress the immune system during infection with S.japonicum and are associated with chronic egg granuloma.更多还原