【摘要】 目的建立一种快速廉价的检测空肠弯曲菌的实时荧光定量PCR方法。方法根据空肠弯曲菌flaA、gyrA基因设计引物,建立空肠弯曲菌实时荧光定量PCR检测方法。结果以flaA基因引物对空肠弯曲菌DNA进行实时荧光定量PCR,扩增曲线呈S形指数增长,大肠埃希菌等对照菌扩增阴性。方法的灵敏度为10CFU/ml菌悬液。结论建立的空肠弯曲菌荧光定量PCR检测方法具有快速、简便、准确等特点,具有一定的使用价值。更多还原

【Abstract】 Objective To establish a rapid and accurate method for detection of Campylobacter jejuni by real-time fluorescent PCR assay.MethodsAccording to the open reading frame (ORF) of sequence of flaA gene, gyrA gene, several couples of specific primers were designed.Reaction system and determination approach of real-time fluorescent PCR were established for detection of C. jejuni.ResultsThe flaA primer pairs were used in real-time PCR assay for detection of C. jejuni, the fluorescent amplification curves were observed as a typical "S" curve, other bacteria were also tested and no amplification was observed.It was found that the sensitivity of this assay was 10 CFU/ml.ConclusionThis real-time PCR assay is fast, simple, sensitive and specific for the purposes of detection of C. jejuni.更多还原