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贵州省2007~2009年度区试小麦品系的SSR分析

Genetic Diversity of Wheat Lines from Guizhou Field Trial of 2007-2009 Revealed by SSR Markers

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【作者】 任明见李吉徐如宏王金洪彭义

【Author】 REN Ming-jian1,LI Ji1,XU Ru-hong1,WANG Jin-hong2,PENG Yi2(1.College of Agricultural,Guizhou University,Guiyang 550025,China;2.Seed Administration Station of Guizhou Province,Guiyang 550025,China)

【机构】 贵州大学农学院贵州省种子管理站

【摘要】 采用微卫星分子标记(SSR)对贵州省2007~2009年参加区域试验的20个小麦品种(系)的遗传多样性进行了研究。结果表明,12对引物共检测到46个等位位点,每对引物等位位点数在2~7之间,平均为3.83个。聚类分析表明,品种间遗传距离(D)变异范围为0.116~0.526,平均值为0.321,SSR标记能将全部材料区分开来,20个小麦品系可分为5类。本研究表明,采用核心引物进行不同小麦新品种的遗传多样性分析,对小麦新品种的特异性检测和品种保护是一种有效的手段。

【Abstract】 The genetic diversity among 20 wheat lines,which had been taken part in Guizhou province field trial in 2007-2009,was investigated by using SSR markers.The results showed that a total of 46 allelic loci were detected by a set of 12 primers.The number of alleles per primer pair ranged from 2 to 7,with average 3.83.Cluster analysis showed the average GD of the wheat varieties breeding was 0.321,ranging from 0.116 to 0.526.The GD among 20 wheat lines was calculated to 5 types based on SSR markers.The results indicated that it was an effective method of specific detection and variety protection using different wheat new variety of genetic diversity with core-primers.

【基金】 贵州省小麦“十一五”攻关项目(黔科合NY字[2006]3002);贵州省农业动植物育种专项(黔农育专字[2007]03、[2008]02);贵州省科技成果重点推广计划项目资助(黔科合成字[2008]5022)
  • 【分类号】S512.1
  • 【被引频次】5
  • 【下载频次】66
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