【摘要】 [目的]探讨p53Arg72Pro多态性与HPV相关宫颈癌发生机制的关系。[方法]采用PCR技术检测210例宫颈癌和95例正常宫颈组织的HPV16DNA,采用免疫组化方法及TUNEL检测p53Arg72Pro三种基因型中p53、p21、Bax、Ki-67蛋白(PI)表达以及细胞凋亡(AI)。[结果]宫颈癌HPV16阳性率为70.5%,与正常宫颈组织(7.4%)相比差异具有统计学意义(P<0.05)。HPV16阳性的宫颈癌中:①p53蛋白阴性和弱阳性表达率(73.6%)高于强阳性率(26.4%),其中p53Arg的阴性表达率(39.2%)高于p53Pro(16.7%),差异有统计学意义(P<0.05);②p21蛋白阴性和弱阳性组中,p53Pro型中PI高于p53Arg型,差异有统计学意义(P<0.05);③Bax蛋白阴性和弱阳性组中,p53Pro型中AI低于p53Arg型,差异有统计学意义(P<0.05)。[结论]p53蛋白可被HPV16E6蛋白降解,其中p53Arg蛋白更易被降解;p53Arg和p53Pro蛋白被降解后,两者抑制细胞增殖能力的降低和诱导细胞凋亡能力的降低程度不同,其中p53Pro蛋白转录激活p21和Bax基因的功能及细胞周期阻滞作用的降低更明显。更多还原

【Abstract】 [Purpose] To explore the relationship between p53Arg72Pro polymorphisms and carcinogenic mechanism in HPV-associated cervical carcinoma.[Methods] HPV16 DNA was determined by PCR and expressions of p53,p21,Bax,Ki-67 protein(PI)and cellular apoptosis(AI)were detected by immunohistochemistry and TUNEL in dif-ferent genotypes of p53Arg72Pro of 210 cases with cervical carcinoma and 95 cases with normal cervical tissue.[Results] The positive rate of HPV16(70.5%)in cervical carcinoma was significantly higher than that in normal cervical tissue(7.4%)(P<0.05).Among patients with HPV positive cervical carcinoma,①the expression rate of p53 weak positive and negative(73.6%)was significantly more than those of p53 strong positive(26.4%)and the negative rate of p53Arg(39.2%)was significantly higher than that of p53Pro(16.7%)(P<0.05);②in the weak positive and negative group of p21 protein expression,the PI of p53Pro was significantly higher than that of 53Arg(P<0.05);③in the weak positive and negative group of Bax protein expression,the AI of p53Pro was significantly lower than that of p53Arg(P<0.05).[Conclusions] p53 protein may be degraded completely or partly by HPVE6 protein,by which p53Arg protein may be easier to be degraded.When p53Arg and p53Pro are degraded,there are significantly differ-ences in the degree of inhibiting cellular proliferation and inducing cellular apoptosis,and the function of p53Pro protein would be decreased significantly in transcripting p21,Bax gene and blocking cell cycle.更多还原