【摘要】 [目的]探讨非甾体抗炎药赖氨匹林(Aspisol)对小鼠黑色素瘤细胞株B16的增殖抑制作用及其可能的机制。[方法]应用MTT比色法分析细胞生长抑制作用,流式细胞技术测定凋亡率,应用Western blot分别检测COX-2蛋白和凋亡相关蛋白Bcl-2、Bax的表达。[结果]2～8mmol/L赖氨匹林可抑制B16细胞的生长,且其作用具有剂量和时间依赖性(P<0.01)。赖氨匹林(2、4、8mmol/L)作用24h后诱导B16细胞的凋亡率分别为5.05%±1.23%、9.25%±1.46%、14.80%±1.98%,并呈浓度依赖性;与对照组(0.18%±0.13%)比较差异有显著性(P<0.01)。赖氨匹林抑制B16细胞COX-2蛋白的表达(P<0.05);赖氨匹林可促进Bax蛋白表达(P<0.05),但抑制Bcl-2的表达(P<0.05)。[结论]赖氨匹林通过影响COX-2蛋白的表达,调节凋亡相关蛋白表达,抑制B16细胞增殖。更多还原

【Abstract】 [Purpose] To explore the inhibitory effect and mechanism of Aspisol,a kind of non-steroidal anti-in-flammatory drugs(NSAIDs) on the proliferation of melanoma B16 cells.[Methods] The inhibitive effects of Aspisol on B16 cells were observed with the MTT assay.The cell apoptosis was detected by flow cytometry.The COX-2,Bax and Bcl-2 protein expressions were detected by Western blot method.[Results] Aspisol of 2～8mmol/L could inhibit the growth of B16 cells in a time-and dose-dependent manner(P<0.01).The apoptotic rate of B16 cells ex-posured to different concentrations of Aspisol(2、4、8mmol/L) for 24h was 5.05%±1.23%,9.25%±1.46%,14.80%± 1.98%,respectively,in a dose-dependent manner and there was significantly difference compared to control group(0.18%±0.13%)(P<0.01).The expression of COX-2 and Bcl-2 protein in B16 cells was down-regulated(P<0.05) and Bax expression up-regulated by Aspisol(P<0.05).[Conclusion] Aspisol can effect the expression of COX-2 protein and induce the expression of apoptosis related proteins to inhibit the proliferation of B16 cells.更多还原