【摘要】 目的:观察滋阴理气中药左归饮对围绝经期大鼠卵巢血管内皮生长因子(vascular endothelial growth factor,VEGF)及富含半胱氨酸的酸性分泌蛋白(secreted protein acidic and rich in cysteine,SPARC)表达的影响。方法:采用围绝经期大鼠动物模型,分别给予低、中、高剂量左归饮(13.78,20.67,31.00 g.kg-1)灌胃处理8周。用RT-PCR和免疫组化法检测卵巢VEGF mRNA及蛋白的表达,用RT-PCR方法检测卵巢SPARC mRNA的表达水平。结果:围绝经期大鼠卵巢VEGF mRNA的表达明显高于青年组(P<0.01),中、高剂量左归饮可有效降低其表达水平(P<0.01)。围绝经期大鼠卵巢SPARC mRNA的表达明显低于青年组(P<0.01),中、高剂量左归饮可使其表达显著升高(P<0.01)。结论:VEGF和SPARC表达异常可能在卵巢衰老过程中起一定作用。滋阴理气中药左归饮能通过下调VEGF的表达、上调SPARC的表达,从而促进衰老大鼠卵巢的血管生成,延缓卵巢衰老过程。更多还原

【Abstract】 Objective:To investigate the effect of one kind of regulating-qi and Nourishing-yin Chinese herbs Zuoguiyin on the expression of rats ovarian vascular endothelial growth factor(VEGF) and secreted protein acidic rich in cysteine(SPARC) during the period of peri-menopause.Method:The animal models of perimenopause rats were established by natural aging.Perimenopause rats were treated by intragastric administration(ig) with low(13.78 g·kg-1),middle(20.67 g·kg-1) and high(31 g·kg-1) dose of Zuoguiyin for 8 weeks.Expression of VEGF and SPARC mRNA were detected by RT-PCR.Immunohistochemistry was used to evaluate expression levels of VEGF protein.Result:Compared with that in the control group,ovarian expression levels of VEGF mRNA and its protein in rats during peri-menopause increased significantly(P<0.01).Middle and high dose of Zuoguiyin could both down-regulate the expression of VEGF in ovaries of praesenilis rats,and the difference has statistical significance(P<0.01).Expression levels of SPARC mRNA in rat ovaries during peri-menopausal period decreased obviously compared to that in the control group(P<0.01).Middle and high dose of Zuoguiyin could greatly promote ovarian SPARC mRNA expression of praesenilis rats(P<0.01).Conclusion:The abnormal changes of VEGF and SPARC may play an important role in the aging process of ovary.Zuoguiyin,one kind of regulating-qi and Nourishing-yin Chinese herbs,can improve ovarian vascular formation through down-regulating the expression of VEGF meanwhile up-regulating the expression of SPARC.And it may delay aging by this way.更多还原