【摘要】 目的:建立RRLC-UV法同时测定丹参药材中9种酚酸的含量。方法:以3,4-二羟基苯乙酸为内标,采用AgilentZorbax Eclipse Plus C18(2.1 mm×50 mm,1.8μm)色谱柱,以乙腈-0.1%甲酸溶液为流动相梯度洗脱,流速0.3 mL.min-1,检测波长质量286 nm。结果:这9种成分在测定质量浓度范围内与色谱峰面积线性关系良好,相关系数不低于0.999 5。仪器精密度和方法精密度RSD均小于2.4%,9种成分平均回收率在96.7%~102.6%,RSD均小于3.1%(n=3)。结论:该方法简便、快速,结果准确,重复性好,适用于丹参药材的质量控制。更多还原

【Abstract】 Objective: To establish a RRLC-UV method for simultaneous determination of nine phenolic acids in Salvia miltiorrhiza.Method: 3,4-Dihydroxyphenylacetic acid was used as internal standard,and analysis was carried out on an Agilent Zorbax Eclipse Plus C18(2.1 mm×50 mm,1.8 μm) column with acetonitrile-0.1% formic acid aqueous solution as mobile phases in gradient elution.The flow rate was 0.3 mL·min-1,and the UV detector was monitored at 286 nm.Result: All calibration curves showed good linear regression within test ranges(r≥0.999 5);and the overall recoveries were in the range of 98.2%-102.6%,with RSD less than 3.1%(n=3).The overall RSD of precision test were less than 2.4%.Conclusion: The developed method was simple,rapid,accurate and reproducible,and can be used for the quality control of S.miltiorrhiza.更多还原