【摘要】 目的:建立了同时测定不同产地的野菊花药材中木犀草素-7-O-葡萄糖苷、芹菜素-7-O-葡萄糖苷、蒙花苷、木犀草素、芹菜素和刺槐素含量的方法。方法:采用RP-HPLC,C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.05%磷酸水溶液梯度洗脱,检测波长326 nm。结果:木犀草素-7-O-葡萄糖苷、芹菜素-7-O-葡萄糖苷、蒙花苷、木犀草素、芹菜素和刺槐素分别在1.08~21.5 mg.L-1(r=0.9990),0.278~5.57 mg.L-1(r=0.9994),5.58~112 mg.L-1(r=0.999 9),0.521~10.4 mg.L-1(r=0.9995),0.162~3.25 mg.L-1(r=0.9999)和0.288~5.76 mg.L-1(r=0.9998)与峰面积呈良好的线性关系,平均加样回收率(n=9)均在96.34%~104.0%,RSD均小于1.1%,仪器精密度(n=6)RSD均小于1.2%,方法重复性(n=6)的RSD均小于2.6%。结论:该分析方法快速、准确,重现性好,可作为野菊花药材的质量控制方法。更多还原

【Abstract】 Objective: To develop an HPLC method for the determination of luteolin-7-O-glycoside,apigenin-7-O-glycoside,linarin,luteolin,apigenin and acacetin in Flos Chrysanthemi Indici simultaneously.Method: The reversed phase HPLC system consisting of a C18 column(4.6 mm×250 mm,5 μm) and a mixture of acetonitrile and 0.05% phosphate acid with gradient elution as the mobile phase was adopted.The absorbance was monitored at 326 nm.Result: The linear response range was 1.08-21.5 mg·L-1(r=0.999 0),0.278-5.57 mg·L-1(r=0.999 4),5.58-112 mg·L-1(r=0.999 9),0.521-10.4 mg·L-1(r=0.999 5),0.162-3.25 mg·L-1(r=0.999 9) and 0.288-5.76 mg·L-1(r=0.999 8),respectively(n=6).The average recoveries(n=9) of six flavonoids were 96.34%-104.0%.All of RSD of precision(n=6) and repeatability(n=6) were less than 1.2% and 2.6%.Conclusion: The validation data demonstrated that the method was accurate and repeatabie,and canbe ase to measure the six flavonoids in Flos Chrysanthemi Indici.更多还原