【摘要】 目的:探讨大粒车前子中苯乙醇苷类化合物对小鼠骨髓来源树突状细胞(DCs)功能调节的影响,为进一步阐明大粒车前子的免疫调节活性提供依据。方法:采用细胞因子诱导法,从Balb/cj小鼠骨髓细胞贴壁分离获得单核细胞,加入含10%胎牛血清、10μg.L-1重组小鼠粒细胞巨噬细胞集落刺激因子(rmGM-CSF)及重组小鼠白细胞介素-4(rmIL-4)的RPMI1640完全培养基培养,在培养第6天,实验组加入车前子苯乙醇苷类化合物(10,50,100 mg.L-1),对照组加入RPMI 1640或LPS(1 mg.L-1),流式细胞仪检测DCs表面分子CD11c,CD86,MHC II和CD80的表达以及各组DCs吞噬功能的变化。结果:车前子毛蕊花苷和异毛蕊花苷能够提高DCs表面分子CD11c,CD86,MHC II和CD80的表达;空白组DCs的吞噬功能很强(45.39%),车前子毛蕊花苷和异毛蕊花苷组DCs吞噬功能都明显下降(分别为37.27%,30.40%,33.45%和40.15%,37.59%,31.07%)。结论:车前子苯乙醇苷类化合物能促进小鼠骨髓来源的DCs表型及功能的成熟。更多还原

【Abstract】 Objective: To elucidate the immunomodulatory mechanism of phenylethanoid glycosides from the seeds of Plantago asiatica by testing its effects on the maturing of murine bone marrow derived dendritic cells(DCs).Method: Monocytes generated from bone marrow of Balb/cj mouse were cultured for 6 days in complete RPMI 1640 medium containing 10% FBS,rmGM-CSF and rmIL-4.50 mg·L-1 acteoside or isoacteoside was added to cells on day 6 of culture for 24 h.The surface molecules expression level of DCs and their phagocytose ability were analysis by flow cytometry.Result: Both acteoside and isoacteoside could increase the expression of CD11c,CD86,MHC II and CD80 on DCs surface.The ability of unstimulated DCs to uptake FITC-dextran was higher than that of phenylethanoid glycosides or LPS treated DCs.Conclusion: Both acteoside and isoacteoside could induce maturation of murine dendritic cells.更多还原