【摘要】 目的:研究荷S180瘤小鼠灌服中药复方抑瘤饮不同时间点肿瘤内血管生成的动态变化,揭示抑瘤饮体内抗瘤的作用机制。方法:56只BALB/c小鼠随机分为抑瘤饮组和对照组(n=28),每组分为4个亚组(每亚组7只)。右腋皮下接种S180肿瘤细胞24 h后,抑瘤饮组小鼠灌服抑瘤饮,对照组小鼠灌服等量凉开水,每日2次,每次0.5 mL;2组分别于灌服第10,20,30,40天点各处死一亚组小鼠。分离肿瘤制成石蜡包埋切片,免疫组化法检测肿瘤组织内血管生成(CD34染色)、血管内皮生长因子(VEGF)、血管内皮生长因子受体-2(VEGFR-2)和内皮抑素(ES)的动态变化,结果以阳性酶点(positive enzyme dot,PED)表示。结果:抑瘤饮能显著降低肿瘤组织内微血管生成:抑瘤饮组CD34在第10,20,30,40天点PED均低于对照组(分别P<0.05,P<0.01,P<0.01和P<0.01)。抑制VEGF和VEGFR-2的表达:抑瘤饮组VEGF在第10,20,30,40天点PED均低于对照组(分别P<0.05,P<0.01,P<0.01和P<0.01):抑瘤饮组VEGFR-2在相应时间点均低于对照组(分别P<0.05,P<0.01,P<0.01,P<0.01)。促进ES的表达:抑瘤饮组ES在相应时间点与对照组比第10天点PED无明显差异,在第20,30,40天点PED均高于对照组(分别P<0.05,P<0.05和P<0.01)。结论:抑瘤饮体内可通过下调VEGF和VEGFR-2表达,上调ES表达,抑制肿瘤组织内血管生成发挥抗瘤作用。更多还原

【Abstract】 Objective:To investigate the dynamic changes in angiogenesis within the tumor tissue of mice bearing S180 tumor at different day-points of oral administration with a Chinese medicine compound"Yiliuyin"(YLY) and to explore the anti-tumor mechanisms of YLY in vivo.Method:Fifty-six BALB/c mice were divided into YLY group and control group (28 mice/group) and each group was divided into four subgroups (7 mice/subgroup),randomly.After 24 hrs of inoculation with S180 tumor cells subcutaneously in the right axilla,YLY in the mice of YLY group and equal volume of cold boiled-water in the mice of control group were administered orally twice every day,0.5 mL each time.The mice of one subgroup from the two groups apiece were killed at 10,20,30 th and 40 th day-point of oral administration,respectively.The tumors were isolated and were made into paraffin embedded sections.The dynamic changes of the angiogenesis (CD34 staining),vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor-2 (VEGFR-2) and endostatin (ES) in tumor tissue were detected by immunohistochemistry staining,and the results were shown as PED (positive enzyme dot).Result:YLY could remarkably decrease the angiogenesis within tumor tissues.The PED of CD34 in control group at 10,20,30 th and 40 th day-point was 392.86±42.01, 481.49±58.34,386.31±54.91 and 376.69±28.71,and that in YLY group was 334.46±33.38,289.34±39.63,257.09±40.00 and 246.57±36.78,respectively.The PED of CD34 in YLY group at each day-point was lower than that in control group (P<0.05, P<0.01,P<0.01 and P<0.01,respectively).The PED of VEGF in control group at 10,20,30 th and 40 th day-point was 852.63±81.65,1 168.40±96.69,1 292.60±147.54 and 1 124.74±139.64,and that in YLY group was 718.40±94.94,866.54±72.40, 859.31±74.02 and 753.34±72.95,respectively.The PED of VEGF in YLY group at each day-point was lower than that in control group (P<0.05,P<0.01,P<0.01 and P<0.01,respectively).The PED of VEGFR-2 in control group at 10th,20th,30th and 40th day-point was 618.63±59.08,750.09±56.72,684.91±72.86 and 644.06±60.25,and that in YLY group was 523.91±64.66, 449.03±46.85,400.06±60.12 and 339.89±45.39,respectively.The PED of VEGFR-2 in YLY group at each day-point was lower than that in control group (P<0.05,P<0.01,P<0.01 and P<0.01,respectively).The PED of ES in control group at 10th,20th,30th and 40th day-point was 250.26±36.27,298.60±44.41,450.86±38.95 and 398.43±34.19,and that in YLY group was 249.57±40.23, 350.03±40.92,499.40±40.29 and 497.94±42.76,respectively.There was no difference between the two groups at 10th day-point.The PED of ES in YLY group was higher than that in control group at 20,30,40 th day-point (P<0.05,P<0.01 and P<0.01, respectively).Conclusion:YLY could exert the anti-tumor role by down-regulating the expression of VEGF and VEGFR-2, up-regulating the expression orES and inhibiting the angiogenesis within tumor tissue.更多还原