【摘要】 目的:建立用高效液相色谱法测定人血浆中卡铂浓度的方法。方法:采用Ultimate C18色谱柱(4.5mm×250mm,5μm);流动相为乙腈-0.02mol.L-1磷酸二氢钾溶液(0.5:99.5);流速1.0mL.min-1;检测波长230nm。结果:卡铂在0.77～49.07mg.L-1范围内线性关系良好(r=0.998),最低检测质量浓度为0.77mg.L-1,卡铂低、中、高浓度检测的日内、日间RSD分别为1.32%、4.25%、3.13%和2.77%、4.75%、3.30%。结论:该法具有操作简便可靠,准确,稳定性高等特点,适合应用于卡铂的治疗药物监测和药动学研究。更多还原

【Abstract】 OBJECTIVE To develop an HPLC method for the determination of Carboplatin concentratioin in human plasma.METHODS The Ultimate C18 column(4.5 mm×250 mm,5 μm)was used with acetonitrile-0.02 mol·L-1 KH2PO4(0.5:99.5) as mobile phase.Flow rate was 1.0 mL·min-1 and the detective wavelength was 230 nm.RESULTS The calibration curve of carboplatin was linear over the range of 0.77-49.07 mg·L-1(r=0.998).The detection limit was 0.77 mg·L-1.The intra-day and inter-day RSD were 1.32%、4.25%、3.13% and 2.77%、4.75%、3.30%.CONCLUSION This method is simple,precise,stabile and suitable to be applied in therapeutical drug monitoring of carboplatin and its pharmacokinetics studiy.更多还原