【摘要】 目的研究刮筋板Excoecaria acerifclia F.Didr.的化学成分和抗肿瘤活性。方法采用硅胶柱色谱,Sephedex LH-20分离化学成分;根据波谱数据和理化性质确定化学结构;四甲基偶氮唑蓝(MTT)法测定化合物对人肝癌细胞系(human hepatocarcinoma cell line)HepG2的体外抑制作用,用半数抑制浓度(IC50)评价其抗肿瘤活性。结果分离鉴定6个化合物,分别为:秦皮苷(1)、异落叶松脂醇9-O-β-D-葡萄糖苷(2)、(-)-5′-methoxyisolariciresinol 3a-O-β-D-glucopyranoside(3)、没食子酸(4)、isostrictiniin(5)、3-O-阿魏酰基奎尼酸甲酯(6);化合物1,2,4,6对体外培养肿瘤细胞的IC50分别为14.71,26.52,34.91,65.42μmol·L-1;在测定浓度范围内剂量依赖关系良好。结论所有化合物均为首次从该植物中得到;首次研究发现化合物1对HepG2具有一定的抗肿瘤活性。更多还原

【Abstract】 OBJECTIVE To investigate the chemical constituents of Excoecaria acerifclia F. Didr. and their antitumoractivities. METHODS The constituents of EtOAc-soluble portion were isolated and purified by column chromatography. Their structures were identified by the their physicochemical properties and spectral features. The cytotoxicities of the purified compounds were evaluated by MTT method against human cancer cell lines HepG2. RESULTS Six compounds were isolated and identified as fraxin (1), isolariciresinol 9-O- β-D-glucopyranoside (2), (-)-5′-methoxyisolariciresinol 3a-O-β-D-glucopyranoside (3), gallic acid (4), isostrictiniin (5) and 3-O-feruloylquinic acid methyl ester (6). CONCLUSION All compounds were isolated from this plant for the first time. Compound 1 showed inhibitory activity towards HepG2 with IC50 values of 14.71 μmol·L-1.更多还原