【摘要】 利用降落PCR的方法,从蓖麻基因组DNA中克隆毒蛋白A链基因的560bp片段;利用一步法将该基因的2个正义片段和2个反义片段分别与pB I-121质粒连接,构建含该基因的正义重复和反义重复表达载体,为利用共抑制技术抑制蓖麻毒蛋白A链基因表达的研究奠定基础。更多还原

【Abstract】 To clone the RTA gene from genome DNA of Ricinus commol/Lunis L.by touch down PCR.The expected 560bp gene fragment was obtained.Two RTA sense fragment and two RTA antisense fragment was linked to the plasmid pBI-121 respectively by one step.The sense and antisense repeat expression vector were constructed.This result provided a foundation for further research of RTA gene expression by co-suppression technology.更多还原