【摘要】 以基础培养基(查彼培养基+碳酸钙+苹果角质)为营养优化了长柄链格孢(Alternaria longipes)产角质酶的环境和营养条件。结果表明,最佳产酶的环境条件是:培养时间8d,培养温度25℃,pH5.5;通过正交试验确定最佳产酶的营养条件是:蔗糖3%,干酪素1%,吐温-800.3%;酶活高达175.2U/mL,是基础培养基酶活力的11.0倍。更多还原

【Abstract】 Alternaria longipes is the causal agent of tobacco brown spot, a world-wild tobacco disease. Based on Czapek medium supplemented with CaCO3 and apple cutin, the liquid fermentation conditions were optimized in order to acquire higher cutinase activity. Optimum culture time, temperature and pH were 8 d, 25 ℃ and 5.5, respectively; and optimum carbon and nitrogen sources were dextrose and maltose instead of NaNO3. According to this fermentation and orthogonal test, the optimum composition of culture medium included dextrose 3%, casein 1%, Tween-80 0.3%. A 11.0-fold higher cutinase activity was obtained compared to the basal medium and the enzyme activity was enhanced from 15.8 U/mL to 175.2 U/mL.更多还原