【摘要】 目的建立南葶苈子饮片中槲皮素、山奈酚及异鼠李素的含量测定方法。方法采用Diamond-C18色谱柱;甲醇-0.4%磷酸溶液(50∶50)为流动相;流速1.0mL·min-1;检测波长360nm。结果线性范围:槲皮素0.1184～0.8288μg,r=0.9995,山奈酚0.0258～0.1806μg,r=0.9994,异鼠李素0.0444～0.3108μg,r=0.9994;平均回收率:槲皮素99.51%,RSD=2.10%(n=6),山奈酚101.03%,RSD=2.08%(n=6),异鼠李素99.80%,RSD=2.09%(n=6)。结论该法操作简便、准确,重复性好,可用于南葶苈子饮片中槲皮素、山奈酚及异鼠李素的含量测定。更多还原

【Abstract】 Objective The Method of determine the quercetin,kaempferol and isorhamnetin in Descurainia Sophia(L.)Webb ex Prantl processed by HPLC was established.Methods The column was Diamond-C18 Column,the mobile phase was methanol-0.4% phosphoric acid(50∶50),the flow rate was 1.0mL·min-1 and the detection wavelength was 360nm.Results The linear range of quercetin was 0.1184～0.8288μg(r=0.9995),the average recovery was 99.51%,RSD=2.10%(n=6).The linear range of kaempferol was 0.0258～0.1806μg(r=0.9994),the averag recovery was 101.03%,RSD=2.08%(n=6).The linear range of isorhamnetin was 0.0444～0.3108μg(r=0.9994),the average recovery was 99.80%,RSD=2.09%(n=6).Conclusions This method is simple,rapid and accurate with good reproducibility,it is suitable for the quality control of Descurainia Sophia(L.)Webb ex Prantl processed.更多还原