【摘要】 目的:研究肿节风注射液(ZJF)对裸鼠人胃癌SGC-7901移植瘤的抑制作用及诱导细胞凋亡作用。方法:建立裸鼠人胃癌SGC-7901移植瘤模型,分为空白、阳性对照和ZJF高、低剂量(3、1.5g·kg-1)组。给予ZJF14d后,测定胸腺、脾脏指数和外周红细胞、白细胞、血小板数目;计算抑瘤率;观察瘤组织超微细胞形态;检测细胞凋亡变化。结果:与空白组比较,胸腺、脾脏指数和外周血象都无显著变化;ZJF高、低剂量组对裸鼠人胃癌SGC-7901移植瘤的抑瘤率有极显著性差异,分别为42.8%、39.9%;给药后细胞形成一个或多个凋亡小体;ZJF高、低剂量组对瘤组织的凋亡率有极显著性差异,分别是(9.3±0.15)%、(8.18±0.08)%。结论:ZJF可促使细胞出现凋亡小体、诱导细胞凋亡,从而抑制裸鼠人胃癌SGC-7901移植瘤的生长,并对胸腺、脾脏、外周血象无显著影响。更多还原

【Abstract】 OBJECTIVE:To study the inhibitory action of Sarcandra glabra injection (ZJF) on tumor growth of SGC-7901 transplantation tumor in nude mice and its induction on apoptosis. METHODS:SGC-7901 transplantation tumor nude mice model was established then the model mice were assigned to blank,positive control,ZJF high-dose and low-dose (3,1.5 g·kg-1) groups. After treatment with ZJF for 14 days,the indexes of thymus and spleen,numbers of peripheral red blood cells,white cells and platelet were measured; the tumor-inhibition rate was computed; the ultrastructure of tumor tissues was observed,and the apoptosis of implanted tumor cells was observed. RESULTS:As compared with blank group,no significant change was noted in the indexes of thymus and spleen and peripheral hemogram. The differences between high-dose ZJF group and low-dose ZJF group were greatly significant in both the inhibition rate of SGC-7901 transplantation tumor (42.8% vs. 39.9%) and the apoptotic rate of tumor tissues [(9.3±0.15)% vs.(8.18±0.08)%].One or more apoptotic bodies formed after medication. CONCLUSION:ZJF can promote the presence of apoptotic body and induce apoptosis thus inhibiting the growth of SGC-7901 transplantation tumor in nude mice; however,it had little impact on the thymus and spleen and peripheral hemogram.更多还原