【摘要】 目的探讨儿童急性淋巴细胞白血病(ALL)急性期、缓解期(CR)患儿血清蛋白质谱的变化,旨在筛选可用于ALL早期诊断、病情变化监测及预后判断的蛋白质标志物。方法应用蛋白质芯片CM10及表面增强激光解吸/离子化飞行时间质谱(SELDI-TOF-MS)技术获得ALL、正常健康儿童血清蛋白表达指纹图谱,并用Biomarker Wizard和Biomarker Patterns System5.0软件对数据进行分析,建立诊断模型。结果通过检测、分析发现,ALL组与正常对照组比较有显著差异(P<0.05)的蛋白质峰有14个,并获得质荷比(M/Z)为2770.43、7576.2和5288.68的蛋白质在ALL患儿血清蛋白质谱中波峰强度明显升高;进一步利用这3个蛋白质建成的诊断模型,可将ALL与正常儿童正确分组,其正确分组率分别为93%(25/27)和90%(19/21)。ALL完全缓解后血清蛋白质谱中,原高表达的蛋白质明显下调。结论SELDI-TOF-MS蛋白质芯片技术是一种快速、简单易行、用量少和高通量分析方法,能够直接检测出ALL患者血清中特异的蛋白标志物,其对于ALL的早期诊断具有一定的临床意义。更多还原

【Abstract】 Objective To investigate the changes of proteomic spectra in serum from children with acute lymphocytic leukemia(ALL) and complete remission(CR) in order to screen the protein markers that can be applied to acute lymphocytic leukemia in its early diagnosis,monitoring of patients’ conditions and prognosis.Methods The fingerprint expressions of protein chips were obtained by using surface enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS) and CMl0’ proteinchip,27 cases were of acute lymphocytic leukemia(ALL),10 cases of ALL complete remission(ALL-CR) and 21 cases of healthy children as a contrast group.The Biomarker Wizard and Biomarker Patterns System 5.0 were used to analyze the data and a diagnostic model was established.Results Fourteen protein peaks were statistically significant(P<0.05) when a contrast was made between the group of children with acute lymphocytic leukemia(ALL) and a contrast group of healthy children.It was found after further analysis that 3 proteins with mass-to-charge ratio(M/Z) of 2770.43,7576.2 and 5288.68 obviously increased their intensity of the peaks of proteomic spectra in serum of the children with ALL.The diagnostic model,established by detection and analysis of the 3 proteins,was able to classify the group of ALL and the group of the healthy.Correspondingly,the correct classification ratios were 93%(24/27) and 90%(19/21).The proteins that originally were over expressed in serum of ALL were evidently down-regulated after ALL complete remission(ALL-CR).Conclusion SELDI-TOF-LMS proteinchip technology is a quick,easy and practica high throughput analytic method.It can detect several specific markers from ALL sera,which had determinate clinical significance for the early diagnosis of ALL.更多还原