【摘要】 本研究探讨了生长激素(STH)和胰岛素对猪卵母细胞体外成熟及孤雌激活后卵裂的影响。结果表明:在mNCSU-23液中,添加0.15μg/mLSTH组的成熟率(73.83%)和卵裂率(64.76%)显著高于对照组和添加0.01、0.05、0.1、0.2μg/mLSTH组(P<0.05);添加5μg/mL和8μg/mL胰岛素组的成熟率(66.25%、60.64%)显著高于添加0、0.5、2μg/mL及10μg/mL组(P<0.05);添加5μg/mL胰岛素组的卵裂率(61.63%)显著高于添加0、0.5、2、8、10μg/mL组(P<0.05);最佳的联合添加组为0.15μg/mLSTH+2μg/mL胰岛素,其体外成熟率为79.37%、卵裂率为72.31%。更多还原

【Abstract】 This experiment investigated the effects of STH and insulin on porcine oocytes maturation in vitro and cleavage after parthenogenetic activation.The results showed that the maturation rate(73.83%) and the cleavage rate(64.76%) derived from oocytes matured in mNCSU-23 supplemented with 0.15 μg/mL STH were significantly higher than those supplemented with 0.01,0.05,0.1,0.2 μg/mL and control group(P < 0.05).When 5 μg/mL or 8 μg/mLinsulin was added to mNCSU-23,respectively,the maturation rate(66.25%,60.64%) were significantly higher than that of other groups(P < 0.05),and the cleavage rate(61.63%) matured in mNCSU-23 supplemented with 5 μg/mL insulin was significantly higher than that of other groups(P < 0.05).The optimum concentrations for STH and insulin to use together were 0.15 μg/mL and 2 μg/mL,the rate of maturation and cleavage were 79.37%,72.31%,respectively.更多还原