【摘要】 目的评价博尔纳病病毒(Borna disease virus,BDV)实时荧光定量PCR(FQ RT-PCR)试剂盒的各项指标,并了解其实际应用效果。方法使用BDVOL持续感染细胞株、非BDV病毒序列转染的OL细胞、正常的OL细胞,对BDV RT-PCR试剂盒的敏感性、特异性、重复性和稳定性进行评估,同时检测部分临床病人和动物外周血液RNA。结果试剂盒可以检测出的病毒RNA最低浓度为102,相当于1.5个病毒拷贝数。特异性好,无非特异检出。不同批次的试剂盒的检测结果变异系数接近1。加速破坏的试剂盒和正常试剂盒检测结果之间变异系数在2以内。对临床病人检测阳性率为3.6%,对动物检测阳性率为4.4%。结论试剂盒敏感性、特异性、重复性和稳定性均佳,是BDV基础研究、流行病学调查、临床检测的良好工具。更多还原

【Abstract】 Objective To evaluate Borna disease virus(BDV)Fluorescent-Quantitation Real Time PCR kit and study the preliminary application results of the kit.Method The sensitivity,distinctness,repeatability and stability of the kit were tested by BDV and non-BDV infected cells,as well as non-infected controls.Human and animal samples were tested by the kit.Result The lowest quantity of virus RNA detected by the kit was 10 2,which corresponded to 1.5 virus copies.The distinctness was good and no non-borna samples were detected.The coefficient of variation(CV)of different batches of kits was close to 1.The CV between normal kits and the kits treated by accelerating decomposition was less than 2.The morbidities of human and animal detected by the kits were 3.6% and 4.4%.Conclusion All of the evaluated indicators of this kit are excellent and this is an efficient tool in fundamental research,clinical detection and epidemiological investigation of BDV.更多还原