【摘要】 目的研究铜绿假单胞菌群体感应系统信号分子3-氧十二烷酰高丝氨酸内酯(3-O-C12-HSL)对人外周血单核细胞凋亡以及TLR2/4 mRNA表达的影响。方法自健康献血者血液中分离单核细胞进行体外培养,并以0、1、10、50和100μmol/L 3-O-C12-HSL作用,采用MTT法,观察不同浓度的3-O-C12-HSL分别作用对人单核细胞生长增殖的影响。用RT-PCR测定单核细胞TLR2/4 mRNA表达。结果3-O-C12-HSL刺激单核细胞12 h后TLR2/4 mRNA表达降低,且呈剂量依赖关系,TLR2/4 mRNA在100μmol/L时最为显著(0.3494±0.0979,0.3351±0.1121)。3-O-C12-HSL对单核细胞增殖有抑制作用,呈剂量依赖关系,差异有显著性(P≤0.05)。结论3-O-C12-HSL可以激发单核细胞细胞凋亡,下调Toll样受体的表达水平,从而削弱单核细胞对铜绿假单胞菌的应答能力,这可能是铜绿假单胞菌逃逸免疫清除的重要原因之一。更多还原

【Abstract】 Objective To investigate the effect of N-(3-oxododecanoyl)-L-homoserine lactone(3-O-C12-HSL,signaling molecule of Pseudomonas aeruginosa biofilm) on the expression of toll like receptors(TLR) 2/4 mRNA and the apoptosis of monocyte.Method Human monocytes,isolated from the peripheral blood of healthy donors,were cultured with 3-O-C12-HSL in different concentration of 0,1,10,50 and 100 μmol/L for 12 hours;the proliferation capacity of cells was observed by MTT.The expression of TLR2/4 mRNA was observed by reverse transcription RT-PCR.Result After 12 h treatment with 3-O-C12-HSL,TLR2/4 mRNA expression was reduced in a dose-dependent manner;the inhibition of 3-O-C12-HSL was most significant at the concentration of 100 μmol/L(0.3494±0.0979,0.3351±0.1121).The proliferation of these monocyte cells was inhibited in a dose-dependent maner.Conclusion 3-O-C12-HSL can induce the monocyte cells apoplosis,decrease the expression of TLRs,and may contribute to immune evasion of the Pseudomonas aeruginosa.更多还原