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化学发光法测定蓝莓叶黄酮提取物对氧自由基的清除作用

Scavenging oxygen free radicals of flavonoids from blueberry leaves by chemiluminescence

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【作者】 李颖畅吴笳笛孙建华刘丽萍

【Author】 LI Ying-chang1,WU Jia-di2,SUN Jian-hua1,LIU Li-ping1 (1.College of Biology and Food Science,Bohai University,Jinzhou 121000,China;2.College of Animal Husbandry and Veterinary,Liaoning Medical College,Jinzhou 121000,China)

【机构】 渤海大学生物与食品科学学院辽宁医学院畜牧兽医学院

【摘要】 文章用化学发光法研究蓝莓叶黄酮对超氧阴离子自由基(O2-)、羟基自由基(.OH)、过氧化氢(H2O2)的清除能力,并与芦丁进行比较。结果表明:蓝莓叶黄酮对O2-.,.OH,H2O2等活性氧均具有清除和抑制作用且清除作用与浓度呈剂量关系,但蓝莓叶黄酮对O2-.,.OH,H2O2的清除能力不如芦丁。蓝莓叶黄酮清除O2-.,.OH,H2O2的IC50分别为13.46,2.11,2.58μg/mL,蓝莓叶黄酮对.OH的清除能力最强。

【Abstract】 The scavenging ability on oxygen free radicals of flavonoids from blueberry leaves was evaluated by chemiluminescence in models for generation of superoxide anions,hydroxyal radicals and peroxide.The results showed that the flavonoids from blueberry leaves could scavenge superoxide anions,hydroxyal radicals and peroxide.The scavenging ability was also related with the concentration of flavonoids.Flavonoids were less stronger ability on scavenging superoxide anions,hydroxyal radicals and peroxide than rutin.IC50 of flavonoids of from blueberry leaves on scavenging superoxide anions,hydroxyal radicals and peroxide were 13.46,2.11,2.58 μg/mL.

【基金】 渤海大学博士启动基金
  • 【文献出处】 中国调味品 ,China Condiment , 编辑部邮箱 ,2009年12期
  • 【分类号】TQ461
  • 【被引频次】6
  • 【下载频次】366
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