【摘要】 目的:研究中华大蟾蜍皮的水溶性成分。方法:利用反复硅胶柱色谱、Sephadex LH-20 凝胶柱色谱、重结晶等手段分离纯化蟾皮的水溶性化学成分, 根据化合物的理化性质和光谱数据鉴定化合物的结构。结果:分离得到7个化合物分别鉴定为蟾蜍环酰胺D(bufogargarizanine D,1), 脱氢蟾蜍噻咛(dehydrobufothionine, 2),环(脯氨酸-甘氨酸)二肽(cyclo(Pro-Gly)dipeptide,3),环(丙氨酸-丙氨酸)二肽(cyclo (Ala-Ala)dipeptide,4), 尿嘧啶(uracil, 5), 胸腺嘧啶(thymine,6),腺苷(gland glucoside,7)。结论:化合物1为新化合物,化合物3-7为首次从中华大蟾蜍皮中分离得到。更多还原

【Abstract】 AIM:To study the water-soluble constituents of the skin of Bufo bufo gargarizans Cantor. METHODS: Repeated silica gel column chromatography, Sephadex LH-20 column chromatography and recrystallization were used to isolate and purify the constituents. Their physico-chemical properties and the spectral analysis were used to identify the structures. RESULTS: Seven compounds were isolated and identified, their structures are bufogargarizanine D (1), dehydrobufothionine (2), cyclo (Pro-Gly) dipeptide (3), cyclo (Ala-Ala) dipeptide (4), uracil (5), thymine (6) and gland glucoside (7). CONCLUSION: Compound 1 is a new compound and compounds 3-7 were isolated from the skin of Bufo bufo gargarizans Cantor for the first time.更多还原