【摘要】 经鉴定和调查,从25个玉米品种中选择抗病性较稳定的14个品种为材料,其中6个品种包括病、健株,共20个样品,进行抗病遗传多样性的RAPD分析。结果表明,不同品种的抗性遗传背景丰富,20个样品经PCR扩增聚类后,以遗传距离15划分,可分为7个类群,同一类群内的品种在抗病性方面有较强的相似性;同一品种的健株与病株间能产生特异性条带,且存在一定的遗传距离,说明植株感病后,其遗传特性会发生改变。田间试验表明,遗传特性差异大的感病品种与抗病品种轮作对顶腐病有较高的防效,可一定程度缓解生产中倒茬难而造成病害流行的难题。更多还原

【Abstract】 14 different resistant varieties were selected from a set of 25 maize varieties via identification and survey. The RAPD analysis for the 14 varieties, 20 samples (6 varieties including infected and healthy plant), was conducted via PCR amplification. The results show that maize varieties are genetically highly diverse. The 20 samples are divided into 7 lineages based on DNA label number at a rescaled distance cluster combination of 15. The variety resistances are highly similar within same lineages. Regarding inheritance, the 6 infected plants (infected by Fusarium subglutinas) are quite different from the healthy ones of same varieties. Field experiments show that rotation planting resistant and susceptible varieties has a significant controlling effect on the occurrence of maize top-rot disease. This resolves the problem of serious maize top-rot disease in continuous planting of maize for several years.更多还原