【摘要】 目的检测链脲佐菌素诱导的DM大鼠模型膀胱乙酰胆碱受体mRNA表达水平的变化及胰岛素治疗的影响。方法实验大鼠分为正常对照组（NC组）、DM模型组（DM组）和胰岛素治疗组（DM+Ins组）。成模10周后取膀胱提取RNA,RT-PCR半定量分析3组大鼠膀胱乙酰胆碱M₂和M₃受体mRNA。结果与NC组相比,DM组和DM+Ins组M₂受体mRNA表达上调显著并以DM组最多,差异有统计学意义（P<0.01）。DM组和DM+Ins组M₃受体mRNA表达下调较NC组显著（P<0.01）,尤以DM组明显（与DM+Ins组比较,P<0.05）。结论 DM大鼠膀胱M₂受体mRNA表达增加,M₃受体mRNA表达则减少,胰岛素治疗可能预防或减轻上述变化。更多还原

【Abstract】 Objective To study the effects of insulin treatment on mRNA expressions of M₂ and M₃ acetylcholine receptors in the urinary bladder wall of diabetic rat.Methods Diabetic SD rat were induced by streptozotocin.The mRNA of M₂ and M₃ acetylcholine receptors in the rat bladder wall were measured with RTPCR at the end of 10^th week.Results Compared with control group（1.13±0.06）,the M₂ mRNA was highest in diabetic group（3.86±0.21） and higher in diabetic group with insulin treatment （1.58 ± 0.11） （P< 0.01）. And compared with control （0.92±0.04） the M₃ mRNA was lowest in diabetic group（0.26±0.01） and lower in diabetic group with insulin treatment （0.28 ± 0.01） （P < 0.01）.Conclusions Diabetic rat expresses the higher M₂ mRNA and lower M₃ mRNA,and the expressions can be reduced by insulin treatment.更多还原