【摘要】 目的探讨血糖控制对新诊断T2DM患者外周血单核细胞（PBMNC）中核因子κB（NF-κB）活性及血清炎症指标的影响。方法 96例新诊断T2DM患者,观察治疗前及血糖良好控制后PBMNC中磷酸化NF-κBp65(Ser⁵³⁶)、血清炎症指标及FFA等变化。结果在血糖控制达标2周时,PBMNC磷酸化NF-κBp65(Ser₅₃₆)、FFA及血清3项炎症指标水平比治疗前显著降低。△hsC-RP与A2hPG、△糖化血清蛋白（FA）呈正相关;与△HDL-C呈负相关;△IL-6与△TC、△LDL-C呈正相关（P<0.05或P<0.01）。结论新诊断T2DM患者在严格控制血糖后,血清hsC-RP、IL-6和TNF-α水平以及PBMNC中NF-κB磷酸化p65(Sers⁵³⁶)水平均明显降低。血糖改善带来炎症状态的改善。更多还原

【Abstract】 Objective To investigate the changes of levels of NF-κB activity in peripheral blood mononuclear cell （PBMNC） and of serum inflammation markers including hs-CRP, TNF-α and IL6 after glycemic control in newly diagnosed type 2 diabetes（T2DM）. Methods 96 newly diagnosed type 2 diabetes patients were selected. Inflammation makers were assayed at 0 weeks and 2 weeks after glycemic control. Serum hs-CRP, TNF-α, IL-6 were assayed by ELISA. Phosphorylation status of NF-κB p65 in PBMNC was determined by immunoblotting method with phosphor-specific antibodies to NF-κB p65 (Ser⁵³⁶). Results Significant decreases in the levels of phosphorylation NF-κB P65(Ser⁵³⁶) and of serum hs-CRP, TNF-α, IL-6 and FFA were found at 2 weeks after glycemic control. Δhs-CRP was positively correlated with Δ2hPG and ΔFA significantly（P<0.05-0.01）, but negatively correlated with ΔHDL-C significantly （r=-0.305,P=0.016）.ΔIL-6 was positively correlated with ΔTC and ΔLDL-C significantly （P<0.05-0.01）. Conclusions The levels of serum hs CRP, IL-6 and TNF-α and the level of phosphorylation NF-κB P65 are decreased at 2 weeks after glycemic control. The improvement of serum glucose contributes to the improvement of inflammation states.更多还原