【摘要】 用2006~2007年从现地采集的疑似感染鸡马立克病(MD)鸡羽髓分别接种鸭胚成纤维细胞(DEF),蚀斑克隆纯化后,随机选1个单克隆,再接种DEF增殖,获得9株适应DEF的鸡马立克病病毒(MDV)野毒株,分别命名为ZY、FY、SY、YC、CZ、MS、CGZ、QQHE和DH。应用PCR技术分别扩增这9株MDV的132 bp重复序列(132 bpr),发现这9株132 bpr均为2个拷贝,符合致病型MDV的特点。从分离的9株MDV中选择5株,人工感染7日龄SPF白来航鸡。试验鸡表现精神萎靡,被毛凌乱,个体瘦小;60d后剖杀,大多可见内脏肿瘤;SY、FY、QQHE 3株的MD临床阳性率达100%。更多还原

【Abstract】 Feather tips of the diseased chickens from nine commercial chickens infected doubtfully with Marek’s disease virus(MDV) from 2006 to 2007 were collected and inoculated into monolayer of duck embryo fibroblast(DEF).Nine MDV isolates ZY,FY,SY,YC,CZ,MS,CGZ,QQHE and DH were identified by plaque clone.The genomic 132 bp repeat sequence of these isolates were analyzed by PCR.All genomes of the isolates contained two copies of the repeat sequence,and resembled the 132 bp repeat sequence of virulent MDV.To examine the pathogenicity of these isolates to chickens,five of the nine isolates were inoculated to 7-day-old white Laihang SPF chickens,respectively.On day 60 post-inoculation,all the survival chickens were euthanaized and necropsied.Most of the chickens with gross MD lesions had visceral tumour.Morbidities of the chickens infected with the inoculated strains SY,FY,and QQHE were 100%,respectively.更多还原