The effects of measuring frequency on the establishment of standrd curve during rapid detetion of tatol becterial number in milk were studied using impedance spectroscopy method. Based on impedance spectroscopy measurement techmique, the change curves of impedance model value |Z| decrease radio with time were compared. At different measuring frequency for pure milk sample with different bacterial numbers (101~108 cfu/mL). In this study, the measuring frequency out of 10kHz~500Hz were unfit for rapid detecti...