【摘要】 为优化纳豆芽孢杆菌固态发酵培养基,采用微生物固态发酵技术,以固态发酵后的活菌数、芽孢数、蛋白酶及α-淀粉酶活力为指标,对纳豆芽孢杆菌固态发酵培养基组成及配比,添加碳源、氮源种类及浓度,培养基的含水量进行优化研究。试验结果表明,在玉米粉与麦麸配比3∶7、葡萄糖2%、硝酸铵0.5%、水60%条件下发酵效果最好。更多还原

【Abstract】 According to the total bacterium spores, the activity of α-amylase and protecase, the solid fermentation culture medium of Bacillus natto was optimized by solid fermentation technology. Two best solid fermentation culture medium was selected from cornflour, wheathusk, soybean residue and soybean meal. The ratio of the two was also selected. The carbon source, the nitrogen source and their concen tration were optimized with the same standards. At last, the amount of water added into culture medium was also optimized. The best culture medium was as follows: the ratio of cornflour to wheathusk 3∶7, glucose2%, ammonium nitrate 0.5%, water60%.更多还原