【摘要】 目的:在慢性水杨酸(SA)耳鸣模型上,观察耳聋左慈丸对大鼠下丘外侧核(ICx)和次听皮层(AII)放电的影响,探讨耳聋左慈丸防治耳鸣的神经机制。方法:健康SD大鼠30只,随机平均分成三组:正常对照组、慢性SA耳鸣模型组和耳聋左慈丸防治组。采用立体定位技术及细胞外记录方法,观察不同组别大鼠ICx和AII自发放电活动,用平均自发放电率和放电间隔直方图为观察指标。结果:①慢性SA耳鸣模型组与正常对照组大鼠相比,ICx神经单元平均自发放电率增高(4.57±0.54Hzvs3.14±0.40Hz,P<0.05),进一步从放电间隔直方图来分析,短间隔自发放电脉冲数占总放电数比例较高(0～40ms为58%vs40%;0～4ms为9%vs5%)。慢性SA耳鸣模型组AII神经单元平均自发放电率与正常对照组大鼠相比有增高趋势(3.84±0.36Hzvs3.17±0.34Hz),短间隔自发放电脉冲数占总放电数比例较正常对照组增高(0～22ms:31%vs16%;0～8ms:19%vs16%)。②耳聋左慈丸防治组大鼠与慢性SA耳鸣模型组相比,ICx和AII神经单元平均自发放电率显著降低(ICx:2.41±0.21Hzvs4.57±0.54Hz,P<0.01;AII:2.24±0.24Hzvs4.57±0.54Hz,P<0.01),短间隔自发放电脉冲数占总放电数比例较低(ICx0～40ms:50%vs58%;0～4ms:4%vs9%;AII0～22ms:24%vs31%;0～8ms:11%vs19%)。结论:慢性SA耳鸣模型动物ICx和AII神经元自发放电活动增加,短间隔放电脉冲数比例较正常对照组增加,耳聋左慈丸能减弱这种变化。更多还原

【Abstract】 Aim:Observe the effects of Er-Long-Zuo-Ci-Wan(ELZCW,a compound of Chinese Traditional Medicine) on the spontaneous discharge of external cortex of inferior colliculus(ICx) and secondary auditory cortex(AII) of chronic tinnitus model rats induced by salicylate acid,to explore the neural mechanisms underlying ELZCW preventing tinnitus. Methods:30 adult SD rats were involved and divided into three groups,normal control group,chronic tinnitus model group and ELZCW prevention group. Extracellular recording techniques and stereotaxic method were used. The spontaneous spikes were recorded and analyzed from ICx and AII in different group rats. The average rate of spontaneous discharge and the interspike interval histogram of spontaneous activities were used as indexes. Results:①Compared with normal control group,the average rate of spontaneous discharge recorded from the ICx in the chronic tinnitus model group increased significantly (4.57±0.54 Hz vs 3.14±0.40 Hz,P<0.05). Furthermore analysis showed that the discharge rate of short spike interval from the ICx in the chronic tinnitus model group increased than that of the normal group(0-40 ms:58% vs 40%;0-4 ms:9% vs 5%). And there was an increasing tendency of the average rate of spontaneous discharge recorded from the AII in the chronic tinnitus model group compared with that in the normal group. ②Compared with the chronic tinnitus model group,the average rate of spontaneous discharge recorded from the ICx and AII in the ELZCW prevention group significantly decreased than that in the chronic tinnitus model group (ICx :2.41±0.21 Hz vs 4.57±0.54 Hz,P<0.01. AII:2.24±0.24 Hz vs 4.57±0.54 Hz ,P<0.01). And the discharge rate of short spike interval from the ICx and AII in the chronic tinnitus model group decreased than that in the normal control group(ICx:0-40 ms 50% vs 58%,0-4 ms 4% vs 9%. AII:0-22 ms:24% vs 31%,0-8 ms 19% vs 16%). Conclusion:If the increasing of the spontaneous activities of ICx and AII in chronic tinnitus rats means tinnitus,the use of ELZCW could decrease this kind of changes.更多还原