【摘要】 目的:观察脚掌皮下注射第三组代谢型谷氨酸受体(mGluRs)激动剂L-SOP对福尔马林(Formalin)炎性痛大鼠脊髓有丝分裂原激活蛋白激酶p38族(p38MAPK)活化的影响。方法:雄性Wistar大鼠48只,随机分为4组(n=12):分别为对照组即生理盐水组和三个不同剂量的L-SOP实验组,每组6只测定痛行为反应,另外6只测定p38MAPK的含量及活化情况。结果:与对照组比较,三个实验组大鼠第二时相的痛行为反应明显被抑制,12.5mmol/L组与25 mmol/L组还观察到大鼠第一时相的痛行为反应亦受到抑制;此外,12.5 mmol/L组与25 mmol/L组脊髓p38MAPK的活化水平与对照组比较明显下降。结论:外周L-SOP能抑制痛行为反应及降低脊髓p38MAPK的活化水平,提示外周组织中有功能性第三组代谢型谷氨酸受体的表达并参与外周痛感受器对伤害性信息的处理。更多还原

【Abstract】 Aim: To explore the effects of periphery injection of L-SOP on the activation of p38MAPK in spinal cord in formalin pain model in rats.Methods: Fourty-eight male Wistar rats were divided randomly into four groups(n=12): NS group and three different dose of L-SOP groups.For each group,6 rats used to observe flinching and licking time every as nociception behavior 3 minutes in 1 hour after formalin injected and the other 6 rats used to observe the activation of p38(P-p38) by Western blotting.Results: All the three different groups of L-SOP could inhibit nociception behavior in the tonic phase,and 250 nmol/L and 500 nmol/L groups could suppress not only in the tonic phase but also in the acute phase.250 nmol/L and 500 nmol/L groups could reduce activated or phosphorylated p38MAPK in spinal cord.Conclusion: Periphery injection of L-SOP can reduce nociceptive behavior and phosphorylated p38MAPK in the spinal cord in formalin-induced hyperalgia,it is suggested that there is functional expression of mGluRs Ⅲ in the periphery and is involved in the processing of peripheral noxious informations.更多还原