【摘要】 目的:探讨运动干预对大鼠骨骼肌应激激活蛋白激酶-JNKmRMA表达、蛋白总量(t-JNK)及磷酸化JNK(p-JNK)的影响。方法:SD大鼠随机分为对照组和运动组。运动组分为1 h/d和1.5 h/d组,运动7周。运动结束后分别在24h和48h取材,测定葡萄糖和胰岛素浓度;Western blot法检测骨骼肌t-JNK蛋白表达、p-JNK磷酸化水平; RT-PCR法分析JNKmRNA表达。结果:与对照组比较,运动组胰岛素浓度降低;1 h运动24 h取材和1.5 h运动24与48h取材组p-JNK增加;1.5 h运动组蛋白总量(t-JNK)升高;JNK mRNA表达变化发生在1.5 h运动24 h取材组;结论:运动干预提高大鼠骨骼肌对胰岛素的敏感性,改善JNK磷酸化、蛋白和基因表达。更多还原

【Abstract】 Aim:To investigate the effects of exercise on JNK phosphorylation,protein and gene expression.Methods:Male rats were randomly divided into control and trained groups.The trained rats were submitted to 1 h or 1.5 h of exercise daily and had a fragment of their excised gastrocnemius muscle,24 h or 48 h after the last training session.The train lasted for 7 weeks.The changes in the expressions of JNK and p-JNK were determined by Western blotting.The expression of JNKmRNA was determined by RT-PCR.Results:Glucose tolerance test found that blood insulin concentration was decreased with exercise training.Exercise led to a marked increase in p-JNK of trained groups 24 hours after exercise in rats that exercised for 1 hour per day and 24 and 48 hours after the exercise in those that exercised for 1.5 hours per day as compared with controls,and the protein expression of JNK significantly increased 24 and 48 hours after the exercise in rats that exercised for 1.5 hours per day.JNKmRNA was increased by exercise 1.5 h/d,24 h after the last training session.Conclusion:Exercise could increase muscle responsiveness to insulin,improving the total JNK and p-JNK and mRNA expression.更多还原