【摘要】 目的:研究慢性间歇低氧对幼鼠部分脑区p38MAPK的影响。方法:SPF级健康雄性SD幼鼠(3～4周龄)50只,随机分为5组(n=10):间歇低氧2周组(2IH组)、间歇低氧4周组(4IH组)、间歇低氧4周后恢复组(4F组)、对照2周组(2C组)和对照4周组(4C组)。建立慢性间歇低氧幼鼠模型,以RT-PCR法和Western blot法分别测幼鼠海马及前额叶皮层p38MAPK mRNA和磷酸化p38MAPK(p-p38)蛋白的表达。结果:2IH、4IH和4F组幼鼠海马、前额叶皮层的p38MAPK mRNA及p-p38蛋白均明显高于相应对照组(P均<0.05)。结论:慢性间歇低氧可激活幼鼠部分脑区p38MAPK。更多还原

【Abstract】 Aim:To study the effect of chronic intermittent hypoxia on p38MAPK in partial cerebral tissues of weanling rats.Methods:Randomly, fifty male SD rats(3-week-old～4-week-old)were divided into five groups:2-week-CIH group(2IH),4-week-CIH group(4IH),4-week-recovery group(4F),2-week-control group(2C)and 4-week-control group(4C).Intermittent hypoxia model was induced by an intermittent hypoxia cabin.The expression of p38MAPK mRNA and the phosphorylation levels of p38MAPK(p-p38 protein)in the hippocampus and prefrontal cortex were measured by RT-PCR or Western blot respectively.Results:No matter in the hippocampus,or in the prefrontal cortex,the expression of p38MAPK mRNA and p-p38 protein in 2IH,4IH and 4F groups were respectively higher than 2C and 4C groups(P<0.05,respectively). Conclusion:Chronic intermittent hypoxia can activate the p38MAPK in partial cerebral tissues of weanling rats.更多还原