【摘要】 目的:探讨给予牛磺酸预处理对肢体缺血/再灌注(limb ischemia-reperfusion,LI/R)后大鼠肝脏损伤及TNFα、NF-κB表达的影响及意义。方法:采用Wistar大鼠建立LI/R损伤模型,随机分为4组(n=10):对照(C)组,缺血/再灌注(I/R)组,牛磺酸(T)组和牛磺酸+缺血/再灌注(TR)组。比色法测定动物血浆ALT、AST、MDA,肝组织MDA、MPO、DNA裂解率和钙含量,放免法检测血浆及肝组织TNF-α水平;HE染色观察肝脏组织形态改变;免疫组化法观察NF-κB蛋白表达。结果:与C组比较,I/R和TR组各损伤性指标、TNF-α水平均升高,NF-κB蛋白表达增高(P<0.01);但TR组上述各项指标较I/R组显著降低。结论:牛磺酸预处理可减轻大鼠LI/R所致肝脏损伤,降低TNF-α、NF-κB表达。更多还原

【Abstract】 Aim:To investigate the effect of pretreatment with taurine on liver injury changes and the change of tumor necrosis factor alpha and NFκB expression following rats limbs ischemia/reperfusion.Methods:The model of limbs ischemia/reperfusion injury on rats was adopted in the experiment.Wistar rats were randomized into 4 groups(n=10):Control group,T group,I/R group and TR group.Levels of alanine aminotransferase (ALT),aspartate aminotransferase(AST)and MDA in the plasma,MDA,MPO,calcium in liver tissues were measured by colorimetric method.The content of TNF-αin plasma and liver tissues was determined by radioimmunoassay.The morphologic changes were observed with HE staining.The expressions of NF-κBp65 in liver tissues were tested by immuno-histochemistry method.Results:It was found that against the conrol group,the test values of ALT,AST,et al.and expressions of TNF-α,NF-κB increased in I/R group and TR group,but va lues of those in TR group were lower than in I/R group.Conclusion:Taurine can decrease the levels of TNF-αand NF-κB.It can mitigate the liver injury after limb ischemia/reperfusion injury in rats.更多还原