【摘要】 目的探讨体外培养的两株不同转移潜能的乳头状浆液性卵巢癌细胞蛋白表达差异及其意义。方法2008年1月于浙江省肿瘤医院研究所提取卵巢癌细胞株HO-8910和高转移卵巢癌细胞株HO-8910PM的细胞裂解液及培养上清液中的总蛋白,利用弱阳离子交换芯片(WCX2)和表面加强激光解析电离飞行时间质谱(SELDI-TOF-MS)技术分析两者的差异表达蛋白峰。用CiphergenProteinChip3.2.1软件分析芯片结果。结果在细胞裂解液中检出两株细胞差异蛋白峰共11个,其中10个蛋白峰在高转移卵巢癌细胞系HO-8910PM中表达上调,1个在HO-8910PM中表达下调。在两株细胞分泌液中共检出4个差异蛋白峰,均为高转移卵巢癌细胞系表达上调。其中质荷比为3144的差异蛋白峰在两株细胞的细胞裂解液及细胞分泌液中均检出,且都为HO-8910PM表达上调。利用Swiss蛋白数据库检索分子量相匹配的蛋白质发现质荷比(m/z)3144蛋白峰可能为信号传导类蛋白CD24。结论不同转移潜能的卵巢癌细胞存在蛋白表达差异。更多还原

【Abstract】 Objective To detect the distinct protein in two human papillary serous ovarian cancer cell lines with different metastasis potencies in vitro.Methods We respectively distilled total proteins of cell lysis solution and culture supernatant of two human ovarian cancer cell lines (HO-8910,a human ovarian cancer cell line,and its highly metastatic subline HO-8910PM.).Differentially expressed protein profile was analyzed by surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) on weak cation exchanger(WCX2) chips respectively.The protein profile was analyzed by Ciphergen proteinchip software 3.2.1.Results Eleven differentially expressed proteins were found in cell lysis solution between two cells,and of them,ten proteins was up-regulated and one protein down-regulated in HO-8910PM,comparing with that in HO-8910.Additionally,four over-expressed proteins were detected in the culture supernatant from HO-8910PM comparing with that from HO-8910.Interestingly,one differentially expressed protein,3144 at M/Z value,was found up-regulated in both the cell lysis solution and culture supernatant from HO-8910PM.Bioinformatic analysis from the SWISS protein database,we found that 3144 at M/Z protein profile may be one of signal transduction proteins CD24.Conclusion There are distinct proteins in protein profiling between high or low metastatic ovarian cancer cell lines.更多还原