【摘要】 目的探讨Dickkopf-1(DKK-1)、低密度脂蛋白受体相关蛋白-5(LRP-5)和低密度脂蛋白受体相关蛋白-6(LRP-6)在卵巢上皮性癌中的表达和临床意义。方法2002年1月至2008年4月在中国医科大学附属盛京医院采用RT-PCR的方法检测56例卵巢上皮性癌、35例卵巢上皮性良性肿瘤和12例正常卵巢组织中DKK-1mRNA、LRP-5mRNA和LRP-6mRNA的表达情况,分析3种基因与各临床病理参数的关系及其在癌组织中的表达相关性。结果DKK-1mRNA,LRP-5mRNA和LRP-6mRNA在卵巢上皮性癌组织中的表达强度分别为0.90±0.32,0.71±0.27,0.80±0.28;明显高于卵巢上皮性良性肿瘤组织0.58±0.23,0.58±0.29,0.54±0.22和正常卵巢组织0.36±0.29,0.40±0.25,0.32±0.27(P均<0.0001)。DKK-1mRNA、LRP-5mRNA和LRP-6mRNA表达强度与临床分期(P=0.003～0.020)和病理分级(P=0.000～0.028)密切相关。DKK-1mRNA与LRP-5mRNA和LRP-6mRNA在卵巢上皮性癌中的表达强度存在统计学相关性(r=0.341,P<0.0001;r=0.815,P<0.0001)。结论DKK-1、LRP-5和LRP-6mRNA在卵巢上皮性癌中表达增强,其表达强度存在相关联系。DKK-1、LRP-5和LRP-6在卵巢上皮性癌的发生、发展过程中起重要作用。更多还原

【Abstract】 Objective To determine the expression and significance of Dickkopf-1(DKK-1),LRP-5 and LRP-6 mRNA in human ovarian epithelial carcinomas.Methods We used the reverse transcription-polymerase chain reaction(RT-PCR) method to detect the expression of DKK-1,LRP-5 and LRP-6 mRNA in 56 malignant tumors,35 benign tumors,and 12 normal ovaries from January 2002 to April 2008 in the Department of Obstetrics and Gynecology,Shengjing Hospital,China Medical University.The correlation of DKK-1 with LRP-5 and LRP-6 and the association of their mRNA levels with clinicopathological parameters were performed.Results The expression of DKK-1,LRP-5 and LRP-6 mRNA was higher in epithelial ovarian cancers(0.90±0.32,0.71±0.27,0.80±0.28,respectively),as compared with the benign control group(0.58±0.23,0.58±0.29,0.54±0.22,respectively) and normal control group(0.36±0.29,0.40±0.25,0.32±0.27,respectively),(P<0.0001).The expression of DKK-1 was statistically associated with FIGO stage(P=0.020)and differentiation(P=0.028).The expression of LRP-5 was statistically associated with FIGO stage(P=0.003) and differentiation(P=0.028).The expression of LRP-6 was statistically associated with FIGO stage(P=0.000).DKK-1 mRNA expression had correlations with LRP-5/6 mRNA expression(r=0.341,P< 0.0001,r=0.815,P<0.0001).Conclusion DKK1,LRP-5 and LRP-6 mRNA are highly expressed in ovarian carcinomas.They might play an important role in epithelial ovarian carcinogenesis.更多还原