【摘要】 目的:研究重组人促红细胞生成素(rhEPO)对大鼠实验性脑出血(ICH)后脑水肿及缺氧诱导因子(HIF-1α)的影响。方法:实验大鼠随机分为假手术组以及ICH与ICH+rhEPO预处理组,后二者又分为术后第3、6、12、24、48、72小时、第7、14、21天各9小组;全部152鼠共分为19组,每组8只鼠。采用自体血脑内注射法建立ICH动物模型,rhEPO干预组于ICH建模后,每日腹腔注射rhEPO。应用免疫组化方法检测脑出血后不同时间点血肿周边脑组织中HIF-1α蛋白表达情况,并应用干湿比重法测定脑组织含水量。结果:出血后,HIF-1α蛋白阳性表达率随时间进行性增加,并于出血后72h达到高峰,此后HIF-1α蛋白表达的阳性率逐渐下降,脑出血后不同时间点阳性率比较具有显著性差异(P<0.05);脑出血后3d内,脑组织含水量进行性增加,此后逐渐下降,脑出血Epo干预组较脑出血组及假手术组各时间点脑组织含水量低,HIF-1α蛋白阳性率较低。结论:促红细胞生成素能显著降低脑出血后脑组织含水量,减少HIF-1α表达,对脑出血后脑组织有良好的保护作用。更多还原

【Abstract】 Objective:To explore the effects of rhEPO on brain edema and HIF-1α in rats intracerebral hemorrhage(ICH) .Method:A total of 152 rats were randomly divided into sham operation group,and 18 groups of the 3rd,6th,12th,24th,48th,72ndh and 7th,14th,21std after ICH and ICH+rhEPO respectively. There were 8 rats in each group. The model of ICH was established in rats by intracerebral injection of autogenous blood.In ICH +rhEPO groups rhEPO was injected(IP) everyday after ICH. The expressions of HIF-1α in rats brain tissue around hematoma were detected with SP immunohistochemical method. The expressions of HIF-1α were compared with the degree of brain edema at different time points after ICH and ICH +rhEPO.Result:The water contents of brain tissue in ICH+rhEPO group were lower than that in ICH groups. The rates of HIF-1α in ICH+rhEPO groups were less than that in ICH groups.Conclusion:rhEPO can significantly reduce brain edema and the HIF-1α rhEPO can protect the brain after intracerebral hemorrhage.更多还原