【摘要】 为调查我国鸡传染性法氏囊病病毒(IBDV)的流行情况,从我国11个省(市、自治区)采集法氏囊病料,分离鉴定鸡传染性法氏囊病病毒20株,运用RT-PCR方法对分离株的VP2基因进行扩增、测序和序列分析。研究表明,20株分离毒株中有19株具有IBDV超强毒株的分子特征,即222A、256I、294I和299S,与我国IBDV超强毒Gx株的核苷酸同源率在96.7%～99.4%,推导氨基酸同源率在98.2%～100%。遗传进化分析表明,所有分离毒株具有共同的祖先。更多还原

【Abstract】 In this study,complete VP2 genes of 20 IBDV isolats from 11 provinces (city or antomous region) were amplified using RT-PCR and sequenced to determine IBDV molecular epidemiology. The results showed that a majority (19/20) of the isolates had very virulent IBDV (vvIBDV)conserved amino acids:222A,242I,256I,294I and 299S,and only SH-h had P222. Compared to Chinese vvIBDV Gx strain,the homologous rates of nucleotide and amino atid were 97.5% to 99.4% and 98.9% to 100% ,respectively. Using serotype Ⅱ of IBDV as the outgroup, a phylogenetic tree based on VP2 nucleotide sequence was constructed. Our findings demonstrated that all isolates had a similar orgion with Gx strain and the other vvIBDV strains.更多还原