【摘要】 目的探讨自由基清除剂依达拉奉(ED)对脓毒症致ALI的保护作用。方法24只雄性Wistar大鼠随机分成3组:对照组(NS组)、模型组(LPS组)及依达拉奉治疗组(ED组)。LPS组和ED组采用尾静脉注射LPS(10 mg/kg)建立ALI模型,ED组随后立即尾静脉注射依达拉奉(3 mg/kg)。LPS注射6 h后留取肺标本,分别测定肺组织湿/干重比值(W/D)和肺组织匀浆中髓过氧化物酶(MPO)、丙二醛(MDA)和超氧化物歧化酶(SOD)含量,观察肺组织病理改变及肺组织核因子κB(NF-κB)表达情况。结果LPS组肺组织MPO、MDA含量、W/D、肺损伤评分及肺组织NF-κB表达均高于NS组(P均<0.01),肺组织SOD含量低于NS组(P<0.01)。ED组肺组织MPO、MDA含量、W/D、肺损伤评分及肺组织NF-κB表达均低于LPS组(P均<0.01),仍高于NS组(P均<0.01);ED组肺组织SOD含量高于LPS组(P<0.01),仍低于NS组(P<0.01)。结论依达拉奉可以减轻LPS诱导的大鼠ALI,其机制可能与清除ROS,降低了NF-кB信号转导通路的活化,进而减轻炎症级联放大效应有关。更多还原

【Abstract】 Objective To explore the potential protective effect in vivo of Edaravone,a free radical scavenger on model of acute lung injury in rats with sepsis.Methods Twenty-four male Wistar rats were randomly divided into three groups,ie.a control group(NS group),a model group(LPS group),a Edaravone treatment group(ED group).ALI was induced by injecting LPS intravenously(10 mg/kg) in the LPS group and the ED group.Meanwhile the ED group was intravenously injected with Edaravone(3 mg/kg).The NS group was injected with normal saline as control.The lung tissue samples were collected at 6 h after intravenous injection.The wet/dry(W/D) weight ratio of lung tissue was measured.The levels of myeloperoxidase(MPO),malondialdehyde(MDA) and superoxide dismutase(SOD) in lung tissue homogenate were assayed.The pathological changes and expression of nuclear factor-kappa B(NF-κB) in lung tissue were also studied.Results Compared with the NS group,The W/D,pathological scores,NF-κB expression,MPO and MDA levels in the LPS group were significantly higher(all P<0.01),and the level of SOD was apparently lower(P<0.01).The W/D,pathological scores,NF-κB expression,MPO and MDA levels in the ED group were significantly lower than those in the LPS group(all P<0.01) and higher than those in the NS group(all P<0.01).And the level of SOD in lung tissue of the ED group was higher than that in the LPS group and lower than that in the NS group(P<0.01).Conclusions Edaravone has protective effect on ALI rat model.The mechanism may be related to its ability of clearing the reactive oxygen species,inhibiting the activation of the signal pathway of NF-κB and inflammatory cascade.更多还原