【摘要】 目的了解哮喘患者呼出气冷凝液(EBC)中硝酸盐/亚硝酸盐(NO2/NO3)、IL-4和IFN-γ的含量,并观察激素治疗对其水平的影响。方法纳入哮喘患者40例,其中初诊轻、中度非激素治疗组(未治疗组)20例,轻中度慢性持续期激素治疗组20例(治疗组);健康对照20例。收集EBC,测定NO2/NO3、IL-4和IFN-γ含量;检测外周血IL-4、IFN-γ含量。结果哮喘未治疗组EBC中NO2/NO3含量显著高于对照组和治疗组[(48.55±27.37)μmol/L比(24.51±18.22)μmol/L、(36.06±25.13)μmol/L,P均<0.05],而治疗组和对照组无显著差异(P>0.05)。三组血和EBC中IL-4、IFN-γ含量无显著差异(P均>0.05)。结论哮喘患者EBC中NO2/NO3含量增高,吸入糖皮质激素治疗后NO2/NO3含量降低,提示哮喘患者在治疗前气道存在明显的氧化应激。EBC中L-4和IFN-γ含量无显著变化,能否用于反映哮喘患者的炎症状态有待进一步研究。更多还原

【Abstract】 Objective To examine the levels of nitrite/nitrate(NO2/NO3),IL-4 and IFN-γ in exhaled breath condensates(EBCs) in patients with asthma,and to investigate the effect of corticosteroid treatment.Methods Forty patients diagnosed as mild to moderate asthma were recruited.Among them,twenty were newly diagnosed and steroid naive(non-treatment group).The other 20 patients had been treated with corticosteroid treatment(treatment group).Twenty healthy volunteers were enrolled as normal control.EBC samples were taken for measurement of NO2/NO3,IL-4 and IFN-γ levels.Serum IL-4 and IFN-γ concentrations were also measured.Results NO2/NO3 level in EBC of the non-treatment group was significantly higher than that of the normal controls and the treatment group[(48.55±27.37) μmol/L vs(24.51±18.22) μmol/L,(36.06±25.13) μmol/L,respectively,both P<0.05],and no significant difference was found between the treatment group and the normal controls(P>0.05).The IL-4 and IFN-γ levels in both EBC and serum had no significant difference between the three groups(all P>0.05).Conclusion NO2/NO3 in EBCs increases in asthmatic patients,and decreases after corticosteroid treatment.Whether IL-4 and IFN-γ levels can be used for reflecting airway inflammation need further study.更多还原