【摘要】 目的探讨在博莱霉素诱导的肺纤维化中是否发生了上皮细胞-间质转分化(EMT)现象,支气管上皮细胞是否参与了这一过程。方法采用α平滑肌肌动蛋白Cre重组酶转基因小鼠(α-SMA-Cre/R26R双转基因鼠)为实验动物,构建博莱霉素诱导的小鼠肺纤维化模型,进行组织X-gal染色和α平滑肌肌动蛋白免疫荧光检测。结果气管内灌注博莱霉素的转基因小鼠支气管上皮下部分X-gal蓝染明显增多,终末小气道周围和肺血管壁蓝染细胞出现或增多,部分地区出现了支气管周围肺泡上皮细胞(ACE)阳性蓝染和浸润。结论在博莱霉素诱导的肺纤维化小鼠模型中可以观察到EMT现象,气道上皮细胞和肺泡上皮细胞能向间充质细胞转化,参与肺纤维化病理过程。更多还原

【Abstract】 Objective To explore whether epithelial to mesenchymal transition(EMT) occurs in bleomycin(BLM) induced pulmonary fibrosis,and the involvement of bronchial epithelial cells(BECs) in the EMT.Methods BLM-induced peribronchial fibrosis in an α-smooth muscle actin-Cre transgenic mouse(α-SMA-Cre/R26R) was examined by pulmonary βgal staining and α-SMA immunofluorescence staining.Results BLM-treated mice showed significantly enhanced βgal staining in subepithelial areas in bronchi,terminal bronchioles and walls of pulmonary vessels.Some alveolar epithelial cells(AECs) in certain peribronchial areas or even a small subset of BECs were also positively stained,as confirmed by α-SMA immunostaining.Conclusions EMT occurs in BLM-induced peribronchial fibrosis mice.BECs,like AECs,have the capacity to undergo EMT and to contribute to mesenchymal expansion in pulmonary fibrosis.更多还原