【摘要】 采用不同浓度的联苯胺(50,100,200mg/kg)对大鼠进行体内染毒,利用聚丙烯酰胺凝胶电泳法分析了大鼠肝脏线粒体SOD、GSH-Px、COX和Ca2+-ATPase同工酶的表达差异.结果表明,同工酶COX-L和ATPase-L只在50mg/kg低剂量浓度联苯胺处理组中特异表达.分析认为,低浓度联苯胺经体内代谢活化后,对大鼠肝脏线粒体COX-L和ATPase-L的表达具有诱导作用;当联苯胺浓度达到200mg/kg时,对SOD、GSH-Px、COX和Ca2+-ATPase同工酶的表达均产生明显的抑制作用.更多还原

【Abstract】 By injecting different concentrations of benzidine (0, 50, 100, and 200mg/kg) into rats, the effect of benzidine on the expression of SOD, GSH-Px, COX and Ca2+-ATPase in liver mitochondrion were investigated by using polyacrylamid gel electrophoresis. COX-L and Ca2+-ATPase-L were expressed at 50mg/kg of benzidine. The liver mitochondrion was induced to express COX-L and Ca2+-ATPase-L by metabolizing benzidine at low concentration while the expression of the four isozymes was suppressed at higher concentration of benzidine (≥200mg/kg).更多还原