【摘要】 为有效利用化感物质控制水华藻类,实验探讨了亚油酸对铜绿微囊藻的抑制机理.结果表明,采用亚油酸处理铜绿微囊藻后,铜绿微囊藻的叶绿素a含量下降、细胞外液中电导率、OD260增加;细胞O2i、MDA产生增多;SOD酶活力和比活力、谷胱甘肽-过氧化物酶(GSH-Px)酶活力在前4d内应激性升高,SOD同工酶的谱带在亚油酸浓度为0.1mg/L时表达增强.在亚油酸抑藻的实验组中同时加入抗氧化剂维生素C后,O2i的产生减少,藻生物量增加.因此推测亚油酸的抑藻机理可能是通过O2i的产生,引起细胞质膜的膜质过氧化,造成细胞膜通透性的改变,并导致DNA等生物大分子的损伤,从而抑制了叶绿素a以及有关蛋白质的合成,最终使藻细胞死亡与溶解.更多还原

【Abstract】 In order to effectively control water-bloom algae by using allelochemicals,the inhibitory mechanism of linoleic acid on M. aeruginosa was analyzed. After the linoleic acid added in the experimental groups,the chlorophyll a content of algal cells decreased,the electrical conductivity(EC) and the OD260 of algal extracellular fluid increased,the generation of malondialdehyde(MDA) and superoxide anion radical(O2 i ) of the algal cells increased,activities of superoxide dismutase(SOD) ,glutathione peroxidase(GSH-Px) increased at the first 4 days and the expression of SOD isoenzyme enhanced at the concentration of linoleic acid being 0.1mg/L. But when antioxidants vitamin C was added the generation of O2 i decreased,and algal biomass increased. All the results indicated that the mechanisms underlying the algal growth inhibition by linoleic acid involved the O2 i generation that induced the lipid peroxidation of cell membrane,changed the penetrability of cell membrane,and resulted in the damage of DNA and some bio-macromolecules,inhibited the synthesis of the chlorophyll a and some relative proteins,breaking the algal cells and causing them melt in the end.更多还原