【摘要】 实验考察了多环芳烃和氯酚类化合物对黄孢原毛平革菌P450的诱导作用及其P450对生物降解的影响.结果表明,萘、菲、2,4-二氯酚、五氯酚能够诱导该真菌P450,使微粒体P450比浓度达到37～137pmol/mg.在营养限制条件下,菲诱导的P450是营养丰富条件的5.7倍;P450抑制剂的作用使萘、菲、芘、2,4-二氯酚的降解效率下降36%～100%,但是对2,4,6-三氯酚和五氯酚的降解未产生影响.在营养丰富条件下,P450抑制剂的作用使萘、菲、芘、2,4,6-三氯酚的降解效率下降47%～100%,使五氯酚的降解效率增大57%,但对2,4-二氯酚的降解未产生影响.更多还原

【Abstract】 The involvement of P450 in degradation of polycyclic aromatic hydrocarbons and chlorophenols by white rot fungus Phanerochaete chrysosporium was investigated. The significant induction of P450s by naphthalene,phenanthrene,2,4-dichlorophenol and pentachlorophenol were observed. The specific concentrations of microsomal P450s were up to 37～137 pmol/mg in the induced cells. The content of phenanthrene-induced P450 from nutrient-limited culture was 5.7 times higher than that from nutrient-sufficient culture. Piperonyl butoxide(PB) ,a P450 inhibitor,markedly inhibited the degradation of naphthalene,phenanthrene and pyrene in both nutrient-limited and nutrient-rich culture medium. Moreover,in nutrient-limited culture medium,PB inhibited 2,4-diclorophenol degradation but had no effect on degradation of 2,4,6-triclorophenol and pentachlorophenol. In nutrient-rich culture medium,in contrast,PB inhibited 2,4,6-triclorophenol degradation but did not affect 2,4-diclorophenol degradation. The degradation of pentachlorophenol was even elevated with the presence of PB in nutrient-rich cultures.更多还原