【摘要】 目的探讨中国汉族人群肺结核发病与CD209基因-336位点多态性的关系,并分析其在肺结核发病中与环境因素的交互作用。方法采用1∶1配对病列对照设计,用序列特异性引物-聚合酶链反应(SSP-PCR)技术检测167对病例和对照的CD209基因-336位点的基因型分布;对肺结核相关环境因素进行问卷调查,并进行单因素和多因素条件Logistic回归分析。结果AA、GA和GG基因型在病例组和对照组的分布频率分别为21.0%,42.5%,36.5%和35.3%,45.4%,19.1%。单因素条件Logistic回归分析显示,GG基因型在病例组中频率显著高于对照组(OR=1.768,OR95%CI=1.167~2.679,P=0.007)。调整环境因素后,多因素条件Logistic回归分析显示,GG基因型与肺结核发病仍有关联(OR=1.918,OR95%CI=1.234~2.979,P=0.004);GG和GA基因型与卡介苗接种史(P=0.000)以及与吸烟史(P=0.000)对肺结核发病均有正相乘模型交互作用。结论CD209基因-336位点GG基因型可能是中国汉族人的肺结核易感基因型,GG和GA基因型可能与环境因素存在交互作用。更多还原

【Abstract】 ObjectiveTo explore the possible relationship between the-336 G/A single nucleotide polymorphisms in CD209 of Chinese Han population and pulmonary tuberculosis,and the interaction with environmental factors of pulmonary tuberculosis.MethodsA 1∶1 matched case-control study was conducted.The-336 CD209 G/A polymorphisms were detected in 167 pairs of subjects by the method of polymerase chain reaction with sequence specific primer(SSP-PCR) technique.Information on related environmental factors of tuberculosis was collected with a self-designed questionmaire.Univariate and multivariate conditional logistic analysis were conducted.ResultsThe genotype frequencies of AA,GA and GG in case group and control group were 21.0%,42.5%,36.5% and 35.3%,45.5%,19.1%,respectively.The genotype frequency of GG(OR=1.768,95%CI=1.167-2.679,P=0.007)was significantly overrepresented in case group in univariate conditional logistic analysis.The significant association remained after adjusting for environmental factors between genotype GG(OR=1.918,95%CI=1.234-2.979,P=0.004) and the suscepibility to pulmonary tuberculosis.There were interactions of multiplicative model between GG ang GA and Bacille Calmette Guerin(BCG)(P=0.032) or smoking(P=0.000).ConclusionThe-336 CD209 GG genotype might be associated with the susceptibility to pulmonary tuberculosis in Chinese Han population,and there might be interactions between environmental factors and the genotype.更多还原