【摘要】 目的比较结核分支杆菌耐多药菌株与药物敏感菌株在蛋白表达水平的差异,为进一步研究结核杆菌耐药的分子调控机制提供依据。方法利用双向聚丙烯酰胺凝胶电泳(2-DE)分离耐多药结核分支杆菌和结核分支杆菌药物敏感菌株总蛋白质,通过计算机图像分析软件比较分析电泳图谱,切取部分差异蛋白质点进行肽质量指纹图谱分析。结果发现27个蛋白质点具有明显差异,其中耐多药结核分支杆菌有6个蛋白质点明显表达上调。质谱分析发现,6个蛋白质点分别代表的蛋白质为无机焦磷酸酶、多肽脯氨酰基顺反同分异构酶A、延伸因子Tu、420个氨基酸的未知功能蛋白质、双加氧酶和转录调节蛋白(MoxR)。结论耐多药结核杆菌与药物敏感菌株在蛋白表达水平存在明显差异。更多还原

【Abstract】 ObjectiveTo compare the protein expression profiles of multi-drug-resistant Mycobacterium tuberculosis isolate with drug-sensitive isolate,and to provide basis for further study on molecular mechanisms of drug-resistant tuberculosis.MethodsThe bactrerial proteins were analyzed with two dimensional electrophoresis.Computer software was used to compare the two dimensional electrophoretic maps.Some differential protein spots were cut off from the gel and subiected to mass spectrometer(MS) analysis.ResultsTwenty-seven differential protein spots were found between the multi-drug-resistant isolate and the drug-sensitive isolate.Six protein spots were obviously up-regulated in the multi-drug-resistant isolate.MS detection showed the six protein spots were inorganic pyrophosphatase,peptidyl-prolyl cis-trans isomerase A,elongation factor Tu,unnamed protein,dioxygenase and moxR protein.ConclusionProtein expression differences were discovered between multi-drug-resistant Mycobacterium tuberculosis isolate and drug-sensitive isolate.Further study on the functions of these differential expression proteins might be helpful in elucidating the molecular mechanisms of drug-resistant tuberculosis.更多还原